国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2012年
2期
77-81
,共5页
人乳头瘤病毒%病毒样颗粒%分离和提纯
人乳頭瘤病毒%病毒樣顆粒%分離和提純
인유두류병독%병독양과립%분리화제순
Human papillomavirus%Virus-like partical%Isolation and purification
人乳头痛病毒( human papillomavirus,HPV)感染是导致尖锐湿疣、宫颈上皮瘤样病变和宫颈癌的主要原因.鉴于HPV的特殊扩增机制,目前尚不能通过体外培养的传统方法来获得病毒用于疫苗研究.HPV的主要衣壳蛋白L1在体外可自行组装成病毒样颗粒(virus-like particle,VLP),形成的VLP在实验动物和人体中均可诱生高滴度的保护性抗体,是理想的预防性疫苗组分.此文就HPV的流行病学、致病机制及其VLP的制备和应用作一综述.
人乳頭痛病毒( human papillomavirus,HPV)感染是導緻尖銳濕疣、宮頸上皮瘤樣病變和宮頸癌的主要原因.鑒于HPV的特殊擴增機製,目前尚不能通過體外培養的傳統方法來穫得病毒用于疫苗研究.HPV的主要衣殼蛋白L1在體外可自行組裝成病毒樣顆粒(virus-like particle,VLP),形成的VLP在實驗動物和人體中均可誘生高滴度的保護性抗體,是理想的預防性疫苗組分.此文就HPV的流行病學、緻病機製及其VLP的製備和應用作一綜述.
인유두통병독( human papillomavirus,HPV)감염시도치첨예습우、궁경상피류양병변화궁경암적주요원인.감우HPV적특수확증궤제,목전상불능통과체외배양적전통방법래획득병독용우역묘연구.HPV적주요의각단백L1재체외가자행조장성병독양과립(virus-like particle,VLP),형성적VLP재실험동물화인체중균가유생고적도적보호성항체,시이상적예방성역묘조분.차문취HPV적류행병학、치병궤제급기VLP적제비화응용작일종술.
Human papillomavirus (HPV) infectoin is the main etiological fator of condyloma acuminatum,cervical intraepithelial neoplasia and cervical cancer. In view of the special amplification mechanism of HPV,it is difficult to obtain HPV for vaccine research by traditional cultural methods in vitro.HPV virus-like particles (VLPs) can be assembled by HPV L1 major capsid protein self in vitro and induce high titers of protective antibodies in animal models and human beings.They are perfect prophylactic vaccine components.This review discusses epidemiology and pathogenesis of HPV,as well as production and application of HPV VLPs.
