CAJ | 학술논문

目的探讨3种不同玻片厚度的激光共聚焦专用细胞培养皿制备的细胞样品在激光共聚焦显微镜(CLSM)下采集的荧光染色图像差异.方法通过活细胞及固定后细胞荧光染色实验,采用激光共聚焦显微镜观察细胞形态及荧光亮度,检测不同条件下成像的平均荧光强度,考察3种厚度玻片(0.085～0.13、0.13～0.16、0.16～0.19mm)的激光共聚焦专用细胞培养皿之间显微成像的差异.结果在观察活细胞线粒体和固定细胞骨架时,玻片厚度为0.13～0.16 mm的皿荧光成像清晰效果均表现为最好,玻片厚度为0.16～0.19mm的皿次之,玻片厚度为0.085～0.13 mm的皿较差.结论玻片厚度对激光共聚焦显微镜成像效果有明显的影响,玻片厚度为0.13～0.16 mm的培养皿其细胞形态舒展,染色后细胞骨架清晰,荧光强度最强,成像效果最佳.
목적 탐토3충불동파편후도적격광공취초전용세포배양명제비적세포양품재격광공취초현미경(CLSM)하채집적형광염색도상차이.방법통과활세포급고정후세포형광염색실험,채용격광공취초현미경관찰세포형태급형광량도,검측불동조건하성상적평균형광강도,고찰3충후도파편(0.085～0.13、0.13～0.16、0.16～0.19mm)적격광공취초전용세포배양명지간현미성상적차이.결과재관찰활세포선립체화고정세포골가시,파편후도위0.13～0.16 mm적명형광성상청석효과균표현위최호,파편후도위0.16～0.19mm적명차지,파편후도위0.085～0.13 mm적명교차.결론파편후도대격광공취초현미경성상효과유명현적영향,파편후도위0.13～0.16 mm적배양명기세포형태서전,염색후세포골가청석,형광강도최강,성상효과최가.
Objective To investigate the effects of different thickness of cell culture dishes on fluorescent image with confocal microscope.Methods The fluorescent staining experiments of live cells and fixed cells were used to determine the differences among three dishes with different thickness coverslips of 0.085～0.13 mm,0.13～0.16 mm and 0.16～0.19 mm,while the cell appearance,fluorescence lightness and mean of fluorescence intensity were studied with confocal microscope.Results Demonstrated by the results of cytoskeleton staining experiments,the dish with 0.13～0.16 mm thickness coverslip was the best choice for confocal microscope,the dish with 0.16～0.19 mm thickness coverslip was the second one,the dish with 0.16～0.19 mmthickness coverslip was the last one.Conclusion The dish with 0.13～0.16 mm thickness coverslip is the best choice for confocal microscope.On this type of dish,the cytoskeleton is unfolding and clear after staining.The intensity of fluorescence is the strongest,and the imaging effect is the best.