CAJ | 학술논문

目的观察多孔生物玻璃(Bioglass)、β-磷酸三钙(β-tricalcium phosphate,β-TCP)、羟基磷灰石(hydroxyapatite,HA)、β-硅酸钙(β-calcium silicate,β-CS)、α-硅酸钙(α-CS)五种生物陶瓷支架植入家兔肌肉的生物学表现.方法应用添加造孔剂工艺烧结制备得到多孔Bioglass、β-TCP、HA、β-CS 和α-CS支架,通过X线衍射、孔隙率测定、生物力学测定进行特征分析.将五种支架植入家兔背部肌肉中,4、8、12、16周取材,通过X线、Micro-CT、组织学、扫描电镜和能谱分析进行观察分析,并取材与支架接触肌肉进行聚合酶链式反应(polymerase chain reaction,PCR)分析骨形态发生蛋白-2 (bone morpho-genetic protein-2,BMP-2)和BMP-7含量.结果五种支架特征分析表明,抗压强度:Bioglass＞α-CS＞β-CS＞β-TCP＞HA;弹性模量:α-CS＜β-TCP＜HA＜ββ-CS＜Bioglass；降解性:β-CS＞α-CS＞β-TCP＞ Bioglass＞ HA.组织学观察五种支架中均未出现新生骨组织.Bioglass、α-CS、β-CS表面有钙磷层形成,说明具有体内生物活性.16周时,只有β-CS出现BMP-2和BMP-7表达.结论多孔硅酸钙支架具有良好体内生物活性、可降解性,但无体内骨诱导性.
목적 관찰다공생물파리(Bioglass)、β-린산삼개(β-tricalcium phosphate,β-TCP)、간기린회석(hydroxyapatite,HA)、β-규산개(β-calcium silicate,β-CS)、α-규산개(α-CS)오충생물도자지가식입가토기육적생물학표현.방법 응용첨가조공제공예소결제비득도다공Bioglass、β-TCP、HA、β-CS 화α-CS지가,통과X선연사、공극솔측정、생물역학측정진행특정분석.장오충지가식입가토배부기육중,4、8、12、16주취재,통과X선、Micro-CT、조직학、소묘전경화능보분석진행관찰분석,병취재여지가접촉기육진행취합매련식반응(polymerase chain reaction,PCR)분석골형태발생단백-2 (bone morpho-genetic protein-2,BMP-2)화BMP-7함량.결과 오충지가특정분석표명,항압강도:Bioglass＞α-CS＞β-CS＞β-TCP＞HA;탄성모량:α-CS＜β-TCP＜HA＜ββ-CS＜Bioglass；강해성:β-CS＞α-CS＞β-TCP＞ Bioglass＞ HA.조직학관찰오충지가중균미출현신생골조직.Bioglass、α-CS、β-CS표면유개린층형성,설명구유체내생물활성.16주시,지유β-CS출현BMP-2화BMP-7표체.결론 다공규산개지가구유량호체내생물활성、가강해성,단무체내골유도성.
Objective To investigate the in vivo biological performance of 5 porous bioceramic scaffolds,which were bioglass,β-tricalcium phosphate (β-TCP),hydroxyapatite (HA),β-calcium silicate (β-CS) and α-CS,implanted in rabbit dorsal muscle.Methods The 5 porous bioceramic scaffolds were fabricated by adding pore-forming materials and sintering,and then were investigated by X-ray diffraction,porosity mensuration and biomechanics test.The scaffolds were implanted into rabbit dorsal muscle for 4,8,12,16 weeks,respectively.The samples were analyzed by X-ray,Micro-CT,histological analysis,scanning electron microscope (SEM) and energy dispersive spectrometer (EDS).The expression of bone morphogenetic protein (BMP-2) and BMP-7 in the muscle in touch with bioceramic scaffolds were also investigated by polymerase chain reaction (PCR).Results The characteristic analysis of 5 scaffolds showed that the sequence of compressive strength was bioglass＞α-CS＞β-CS＞β-TCP＞HA,the sequence of elasticity modulus was α-CS＜β-TCP＜HA＜β-CS＜bioglass.It was confirmed by X-ray,Micro-CT and histological analysis that the sequence of biodegradability was β-CS＞α-CS＞β-TCP＞bioglass＞HA.The histological observation showed no new bone formation in five scaffolds.A Ca-P layer was formed in the surface of bioglass,α-CS and β-CS,which suggested their in vivo bioactivity.After 16 weeks,the expression of BMP-2 and BMP-7 was found only in β-CS.Conclusion The porous calcium silicate scaffold,which was promising for bone tissue engineering,was with good in vivo bioactivity and biodegradability,without in vivo osteoinductivity.