中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
3期
160-162
,共3页
李州利%蔡明%陈莉萍%张赟%徐竹蔚%金伯泉%石炳毅
李州利%蔡明%陳莉萍%張赟%徐竹蔚%金伯泉%石炳毅
리주리%채명%진리평%장빈%서죽위%금백천%석병의
肾移植%移植物排斥%双单克隆抗体夹心EISA%可溶型CD305
腎移植%移植物排斥%雙單剋隆抗體夾心EISA%可溶型CD305
신이식%이식물배척%쌍단극륭항체협심EISA%가용형CD305
Kidney transplanation%Graft rejection%Double monoclonal antibody sandwichenzyme linked immunosorbent assay%Soluble CD305
目的 探讨抑制性受体CD205的表达在移植肾排斥反应中的意义.方法 应用双单克隆抗体夹心ELISA法检测153例肾移植术后患者血清中可,溶型CD305(sCD305)的表达水平,20例健康志愿者标本作为对照组.结果 对照组和98例移植肾功能正常患者血清sCD305表达分别为(4.3±2.3)和(6.3±3.7)μg/L.20例移植肾急性排斥及5例移植肾失功患者血清sCD205表达明显增加,分别为(36.3±14.7)和(28.8土9.4)μg/L,显著高于对照组和移植物功能正常组(P＜0.01).30例移植.肾慢性排斥及6例尿毒症透析患者血清sCD205分别为(13.1±5.5)和(11.2±4.6)μg/L,亦明显高于对照组和移植肾功能正常组(P=0.00).结论 发生移植肾排斥反应的患者血清sCD3205有较高水平的表达,可望作为移植肾排斥反应的监测指标之一.
目的 探討抑製性受體CD205的錶達在移植腎排斥反應中的意義.方法 應用雙單剋隆抗體夾心ELISA法檢測153例腎移植術後患者血清中可,溶型CD305(sCD305)的錶達水平,20例健康誌願者標本作為對照組.結果 對照組和98例移植腎功能正常患者血清sCD305錶達分彆為(4.3±2.3)和(6.3±3.7)μg/L.20例移植腎急性排斥及5例移植腎失功患者血清sCD205錶達明顯增加,分彆為(36.3±14.7)和(28.8土9.4)μg/L,顯著高于對照組和移植物功能正常組(P＜0.01).30例移植.腎慢性排斥及6例尿毒癥透析患者血清sCD205分彆為(13.1±5.5)和(11.2±4.6)μg/L,亦明顯高于對照組和移植腎功能正常組(P=0.00).結論 髮生移植腎排斥反應的患者血清sCD3205有較高水平的錶達,可望作為移植腎排斥反應的鑑測指標之一.
목적 탐토억제성수체CD205적표체재이식신배척반응중적의의.방법 응용쌍단극륭항체협심ELISA법검측153례신이식술후환자혈청중가,용형CD305(sCD305)적표체수평,20례건강지원자표본작위대조조.결과 대조조화98례이식신공능정상환자혈청sCD305표체분별위(4.3±2.3)화(6.3±3.7)μg/L.20례이식신급성배척급5례이식신실공환자혈청sCD205표체명현증가,분별위(36.3±14.7)화(28.8토9.4)μg/L,현저고우대조조화이식물공능정상조(P＜0.01).30례이식.신만성배척급6례뇨독증투석환자혈청sCD205분별위(13.1±5.5)화(11.2±4.6)μg/L,역명현고우대조조화이식신공능정상조(P=0.00).결론 발생이식신배척반응적환자혈청sCD3205유교고수평적표체,가망작위이식신배척반응적감측지표지일.
Objective To investigate the role of soluble CD305(sCD305)in renal allograft rejection.Methods Concentration of serum sCD305 was detected on 20 healthy volunteers and 153 cases of recipients after kidney transplantation by using double monoclonal antibody sandwich enzyme linked immunosorbent assay.Results In the healthy volunteers and 98 recipients with normal renal funetion,sCD305 was detected at low levels of(4.3±2.3)μg/L and(6.3±3.7)μg/L.In 20 cases of acute rejection and 5 cases of graft loss,serum sCD305 levels were(36.3±14.7)μg/L and(28.8±9.4)μg/L,and significantly higher than those in the healthy volunteers and recipients with normal renal function.Meanwhile,in the 30 cases of chronic rejection and 6 cases under dialysis treatment,the levels of sCD205were(13.1±5.5)ttg/L and(11.2±4.6)μg/L and significantly higher than those in the healthy volunteers and recipients with normal renal function.Conclusions CD305 was presented at high level in the recipients with renal acute or chronic rejection,and it might be a potential marker for monitoring graft rejection after transplantation.
