CAJ | 학술논문

目的观察人前体脂肪细胞诱导分化过程中NYGGF4基因mRNA表达水平的变化,探讨重组肿瘤坏死因子α(tumor necrosis factor α,TNFα)对成熟脂肪细胞中NYGGF4基因表达水平的调节作用.方法体外培养人内脏来源的原代前体脂肪细胞(human preadipocytes-visceral,HPA-v),在诱导HPA-v分化成熟的基础上,应用不同浓度重组TNFα干预成熟脂肪细胞16 h,或以10 ng/mL TNFα分别干预不同时间,采用实时荧光定量逆转录PCR技术检测的NYGGF4 mRNA表达水平.结果 (1)HPA-v诱导分化至第17 d,具备成熟脂肪细胞特征;(2)NYGGF4基因低表达于人前体脂肪细胞中,随脂肪细胞分化成熟其表达水平逐渐升高;(3)随TNFα干预浓度增高及干预时间延长,人成熟脂肪细胞中NYGGF4mRNA水平呈现逐渐升高的趋势.结论 NYGGF4基因具随脂肪细胞分化成熟表达逐渐上调的特征,TNFα能显著上调成熟脂肪细胞中NYGGF4基因的表达,其效应具有剂量依赖和时间反应性.
목적 관찰인전체지방세포유도분화과정중NYGGF4기인mRNA표체수평적변화,탐토중조종류배사인자α(tumor necrosis factor α,TNFα)대성숙지방세포중NYGGF4기인표체수평적조절작용.방법 체외배양인내장래원적원대전체지방세포(human preadipocytes-visceral,HPA-v),재유도HPA-v분화성숙적기출상,응용불동농도중조TNFα간예성숙지방세포16 h,혹이10 ng/mL TNFα분별간예불동시간,채용실시형광정량역전록PCR기술검측적NYGGF4 mRNA표체수평.결과 (1)HPA-v유도분화지제17 d,구비성숙지방세포특정;(2)NYGGF4기인저표체우인전체지방세포중,수지방세포분화성숙기표체수평축점승고;(3)수TNFα간예농도증고급간예시간연장,인성숙지방세포중NYGGF4mRNA수평정현축점승고적추세.결론 NYGGF4기인구수지방세포분화성숙표체축점상조적특정,TNFα능현저상조성숙지방세포중NYGGF4기인적표체,기효응구유제량의뢰화시간반응성.
Objective To observe the changes of NYGGF4 gene expression during human preadipocyte differentiation and to explore the regulative role of tumor necrosis factor α(TNFα) on NYGGF4 gene expression in matured human adipocytes. Methods Human preadipocytes-visceral (HPA-v)were cultured in vitro and differentiated into the matured adipocytes. Fully differentiated adipocytes were treated with TNFα in different concentrations for 16 hours or at 10 ng/mL for various times. Levels of the NYGGF4 mRNA were evaluated by real-time reverse transcription-PCR(RT-PCR). Results (1) After induction of differentiation, more than 90% of the HPAs-v exhibited typical adipocyte morphology on the 17th day. (2) In human preadipocytes, the level of NYGGF4 mRNA expression remained low. The NYGGF4 mRNA level was gradually increased with the preadipocytes being differentiated into the matured adipocytes, and reached the highest level in the fully differentiated adipocytes. (3) Treatment of the human matured adipocytes with TNFα resulted in a significant increase in the level of NYGGF4 mRNA. The upregulatory effect of TNFα on the NYGGF4 gene expression tended to be enhanced with the increasing concentrations and elongation of time. Conclusion During human preadipocytes differentiation, the level of NYGGF4 gene mRNA expression increases gradually. TNFα could upregulate the expression of NYGGF4 mRNA in human matured adipocytes. The regulatory effect of TNFα on the NYGGF4 gene expression is of dose-dependent and time-correlated.