肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
12期
796-798
,共3页
任婷婷%郭卫%卢新昌%杨毅
任婷婷%郭衛%盧新昌%楊毅
임정정%곽위%로신창%양의
骨肉瘤%砷剂%迁移%肿瘤浸润%基质金属蛋白酶9
骨肉瘤%砷劑%遷移%腫瘤浸潤%基質金屬蛋白酶9
골육류%신제%천이%종류침윤%기질금속단백매9
Osteosarcoma%Arsenicals%Migration%Neoplasm invasivenes%Matrix metalloproteinase 9
目的 研究二氧化二砷(As_2O_3)对骨肉瘤细胞运动、迁移和侵袭的影响及初步机制探讨.方法 通过初筛选用人类骨肉瘤细胞系MNNG,采用细胞迁移实验、伤口愈合实验、侵袭实验和免疫印迹等方法研究As_2O_3,对骨肉瘤细胞转移的影响.结果 通过迁移实验选择迁移能力最强的MNNG细胞作为研究对象,经过As_2O_3处理后.MNNG细胞的伤口愈合能力减弱,迁移和侵袭的穿膜细胞数均明显低于对照组,差异有统计学意义(P=0.0016,P=0.0015),并能下调基质金属蛋门酶9(MMP-9)的表达.结论 As_2O_3能有效抑制骨肉瘤细胞的运动、迁移和侵袭能力,其作用机制可能与下凋MMP-9的表达相关.
目的 研究二氧化二砷(As_2O_3)對骨肉瘤細胞運動、遷移和侵襲的影響及初步機製探討.方法 通過初篩選用人類骨肉瘤細胞繫MNNG,採用細胞遷移實驗、傷口愈閤實驗、侵襲實驗和免疫印跡等方法研究As_2O_3,對骨肉瘤細胞轉移的影響.結果 通過遷移實驗選擇遷移能力最彊的MNNG細胞作為研究對象,經過As_2O_3處理後.MNNG細胞的傷口愈閤能力減弱,遷移和侵襲的穿膜細胞數均明顯低于對照組,差異有統計學意義(P=0.0016,P=0.0015),併能下調基質金屬蛋門酶9(MMP-9)的錶達.結論 As_2O_3能有效抑製骨肉瘤細胞的運動、遷移和侵襲能力,其作用機製可能與下凋MMP-9的錶達相關.
목적 연구이양화이신(As_2O_3)대골육류세포운동、천이화침습적영향급초보궤제탐토.방법 통과초사선용인류골육류세포계MNNG,채용세포천이실험、상구유합실험、침습실험화면역인적등방법연구As_2O_3,대골육류세포전이적영향.결과 통과천이실험선택천이능력최강적MNNG세포작위연구대상,경과As_2O_3처리후.MNNG세포적상구유합능력감약,천이화침습적천막세포수균명현저우대조조,차이유통계학의의(P=0.0016,P=0.0015),병능하조기질금속단문매9(MMP-9)적표체.결론 As_2O_3능유효억제골육류세포적운동、천이화침습능력,기작용궤제가능여하조MMP-9적표체상관.
Objective To study the effect and mechanism of arsenic trioxide on the motility, metastasis and invasion of osteosarcoma in vitro. Methods Wound healing assay, migration assay, invasion assay and Western-blot were performed to study the effect of arsenic trioxide on metastasis of osteosarcoma. Results Through screening MNNG cell was selected to perform the following research. After treatment of As_2O_3, the ability of MNNC cell flattening and spreading along the edges of the wound was inhibited, and the number of MNNG cells with migration and invasion in As_2O_3 - treated group was significantly less than in control group. Arsenic trioxide treatment also resulted in down-regulation of MMP-9. Conclusion This study is the first to report the effectiveness of arsenic trioxide as an inhibitor of osteosarcoma migration and invasion and the mechanism may be down-regulation of MMP-9.