中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2010年
9期
617-620
,共4页
凡治国%张连生%柴晔%曾鹏云%吴重阳
凡治國%張連生%柴曄%曾鵬雲%吳重暘
범치국%장련생%시엽%증붕운%오중양
白血病,非淋巴细胞,急性%Th17细胞%IL-17%TGF-β1%IL-17 mRNA
白血病,非淋巴細胞,急性%Th17細胞%IL-17%TGF-β1%IL-17 mRNA
백혈병,비림파세포,급성%Th17세포%IL-17%TGF-β1%IL-17 mRNA
Leukemia,myeloid,acute%Th17 cell%IL-17%TGF-β1%IL-17mRNA
目的 研究Th17细胞在急性髓系白血病(AML)患者外周血中的表达水平,并探讨Th17细胞的改变与疾病的相关性.方法 ①用酶联免疫吸附实验(ELISA)分别检测AML初诊组、化疗完全缓解或部分缓解组及正常对照组外周血中IL-17、TGF-β1的表达水平.②用流式细胞术分别检测AML初诊组、化疗完全缓解或部分缓解组及正常对照组外周血中Th17细胞所占比例.③用半定量RT-PCR分别检测各组外周血中Th17细胞的相关转录因子IL-17 mRNA的表达水平,并逐层分析比较.结果 AML初诊组和治疗后缓解组IL-17水平、Th17细胞比例及IL-17 mRNA的表达水平分别为(10.502±1.071)ng/L、(0.935±0.140)%、0.262±0.510和(11.345±0.987)ng/L、(1.091±0.159)%和0.307±0.031,均明显低于正常对照组的(16.852±1.198)ng/L、(2.586±0.235)%、0.501±0.060(P＞0.01),AML缓解组Th17细胞比例、IL-17及IL-17 mRNA的表达水平均高于AML初诊组,差异有统计学意义(P＞0.05),AML初诊组和缓解组中TGF-β1的表达水平分别为(29.963±1.588)ng/L、(25.163±1.848)ng/L,明显高于正常对照组(13.366±1.565)ng/L(P＞0.01),且AML初诊组TGF-β1的表达水平高于AML缓解组(P＞0.05).结论 Th17细胞可能与AML的发生发展呈负相关,提示AML患者体内TGF-β1的过度表达可能抑制Th17细胞的分化发育.
目的 研究Th17細胞在急性髓繫白血病(AML)患者外週血中的錶達水平,併探討Th17細胞的改變與疾病的相關性.方法 ①用酶聯免疫吸附實驗(ELISA)分彆檢測AML初診組、化療完全緩解或部分緩解組及正常對照組外週血中IL-17、TGF-β1的錶達水平.②用流式細胞術分彆檢測AML初診組、化療完全緩解或部分緩解組及正常對照組外週血中Th17細胞所佔比例.③用半定量RT-PCR分彆檢測各組外週血中Th17細胞的相關轉錄因子IL-17 mRNA的錶達水平,併逐層分析比較.結果 AML初診組和治療後緩解組IL-17水平、Th17細胞比例及IL-17 mRNA的錶達水平分彆為(10.502±1.071)ng/L、(0.935±0.140)%、0.262±0.510和(11.345±0.987)ng/L、(1.091±0.159)%和0.307±0.031,均明顯低于正常對照組的(16.852±1.198)ng/L、(2.586±0.235)%、0.501±0.060(P＞0.01),AML緩解組Th17細胞比例、IL-17及IL-17 mRNA的錶達水平均高于AML初診組,差異有統計學意義(P＞0.05),AML初診組和緩解組中TGF-β1的錶達水平分彆為(29.963±1.588)ng/L、(25.163±1.848)ng/L,明顯高于正常對照組(13.366±1.565)ng/L(P＞0.01),且AML初診組TGF-β1的錶達水平高于AML緩解組(P＞0.05).結論 Th17細胞可能與AML的髮生髮展呈負相關,提示AML患者體內TGF-β1的過度錶達可能抑製Th17細胞的分化髮育.
목적 연구Th17세포재급성수계백혈병(AML)환자외주혈중적표체수평,병탐토Th17세포적개변여질병적상관성.방법 ①용매련면역흡부실험(ELISA)분별검측AML초진조、화료완전완해혹부분완해조급정상대조조외주혈중IL-17、TGF-β1적표체수평.②용류식세포술분별검측AML초진조、화료완전완해혹부분완해조급정상대조조외주혈중Th17세포소점비례.③용반정량RT-PCR분별검측각조외주혈중Th17세포적상관전록인자IL-17 mRNA적표체수평,병축층분석비교.결과 AML초진조화치료후완해조IL-17수평、Th17세포비례급IL-17 mRNA적표체수평분별위(10.502±1.071)ng/L、(0.935±0.140)%、0.262±0.510화(11.345±0.987)ng/L、(1.091±0.159)%화0.307±0.031,균명현저우정상대조조적(16.852±1.198)ng/L、(2.586±0.235)%、0.501±0.060(P＞0.01),AML완해조Th17세포비례、IL-17급IL-17 mRNA적표체수평균고우AML초진조,차이유통계학의의(P＞0.05),AML초진조화완해조중TGF-β1적표체수평분별위(29.963±1.588)ng/L、(25.163±1.848)ng/L,명현고우정상대조조(13.366±1.565)ng/L(P＞0.01),차AML초진조TGF-β1적표체수평고우AML완해조(P＞0.05).결론 Th17세포가능여AML적발생발전정부상관,제시AML환자체내TGF-β1적과도표체가능억제Th17세포적분화발육.
Objective To investigate the proportion of Th17 cells in the peripheral blood of the patients with acute myeloid leukemia(AML) and evaluate the potential association of Th17 cells with AML.Methods The cytokines IL-17 and TGF-β1 in the peripheral blood of AML patients before therapy (group 1), AML patients in complete remission (AML-CR, group 2) and healthy donors (group 3 ) were measured by enzyme-linked immunosorbent assay( ELISA ). The proportion of Th17 cells of each group was evaluated by flow cytometry. The level of IL-17 mRNA of each group was examined by reverse transcription-PCR ( RTPCR). Results The percentage of Th17 cells and the level of IL-17, IL-17 mRNA in group 1 [( 10.502 ±1.071) ng/L, (0.935±0. 140)% and 0.262 ±0.510] and group 2 [(11.345±0.987) ng/L, (1.091 ±0. 159)% and 0. 307 ± 0. 031] was significantly lower than that in group 3 [( 16. 852 ± 1. 198) ng/L,( 2. 586 ± 0. 235 ) % and 0.501 ± 0. 060]. The percentage of Th17 cells and the level of IL-17, IL- 17 mRNA in group 1 was lower than that in the group 2. But the level of TGF-β1 in the group 1 (29.963 ± 1. 588 ) ng/Land the group 2 (25. 163 ± 1. 848) ng/L was significantly higher than that in group 3 ( 13. 366 ± 1.565)ng/L. However, the level of TGF-β1 in the group 3 was higher than that of the group 2. Conclusion Th17cells might be negatively correlated with the AML development. The overexpression of TGF-β1 in AML patients might suppress the differentiation of Th17 cells.
