CAJ | 학술논문

目的观察旋转磁场对急性放射性食管炎模型大鼠的防治作用.方法根据随机数字表法将40只雌性W istar大鼠随机分为正常对照组(对照组)、辐射组、氨磷汀治疗组(氨磷汀组)、90 min磁场治疗组(90 min磁场组)和120 min磁场治疗组(120 min磁场组)五组,每组8只.除对照组外,其余4组用6MVX线直线加速器对大鼠食管上段3 cm单次照射43 Gy制作放射性食管炎模型.每组分别于照射后第7天和第14天两个时间点,各取4只大鼠进行活体观察和取材:取腹主动脉血检测血常规和细胞因子水平；取全长食管组织,常规石蜡包埋,切片,经HE染色,光镜下观察病理改变.结果辐射组大鼠食管组织于照射后第7天出现放射性食管炎改变,食管黏膜上皮坏死脱落,黏膜下层充血、水肿,并伴有大量炎症细胞浸润；照射后第14天病理改变与第7天相似.氨磷汀组、90 min磁场组和120 min磁场组大鼠食管组织放射性炎症反应较轻,且照射后第14天黏膜鳞状上皮有修复倾向,血常规检查发现白细胞和中性粒细胞数明显低于辐射组(P＜0.05)；而90 min磁场组和120 min磁场组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1( IL-1)和白细胞介素-6(IL-6)均较辐射组低(P＜0.05).结论旋转磁场和氨磷汀对大鼠放射性食管炎均有较好的防治作用,疗效相近；旋转磁场可抑制致炎因子的表达,减轻食管组织的急性放射性炎症反应.
목적 관찰선전자장대급성방사성식관염모형대서적방치작용.방법 근거수궤수자표법장40지자성W istar대서수궤분위정상대조조(대조조)、복사조、안린정치료조(안린정조)、90 min자장치료조(90 min자장조)화120 min자장치료조(120 min자장조)오조,매조8지.제대조조외,기여4조용6MVX선직선가속기대대서식관상단3 cm단차조사43 Gy제작방사성식관염모형.매조분별우조사후제7천화제14천량개시간점,각취4지대서진행활체관찰화취재:취복주동맥혈검측혈상규화세포인자수평；취전장식관조직,상규석사포매,절편,경HE염색,광경하관찰병리개변.결과 복사조대서식관조직우조사후제7천출현방사성식관염개변,식관점막상피배사탈락,점막하층충혈、수종,병반유대량염증세포침윤；조사후제14천병리개변여제7천상사.안린정조、90 min자장조화120 min자장조대서식관조직방사성염증반응교경,차조사후제14천점막린상상피유수복경향,혈상규검사발현백세포화중성립세포수명현저우복사조(P＜0.05)；이90 min자장조화120 min자장조대서혈청종류배사인자-α(TNF-α)、백세포개소-1( IL-1)화백세포개소-6(IL-6)균교복사조저(P＜0.05).결론 선전자장화안린정대대서방사성식관염균유교호적방치작용,료효상근；선전자장가억제치염인자적표체,감경식관조직적급성방사성염증반응.
Objective To observe the effect of a rotating magnetic field in preventing and treating irradiation-induced esophagitis in rats. Methods Forty female Wistar rats were randomized into 5 groups:a non-irradiated control group,an irradiation group,an amifostine treatment group ( amifostine group ),a 90 min magnetic field treatment group (90 min magnetic group) and a 120 min magnetic field treatment group ( 120 min magnetic group),with 8 rats in each group.The esophaguses of all rats except those in the control group were exposed to a single irradiation with 6 MV X-rays from a linear accelerator at a dosage of 43 Gy.Four rats in each group were randomly chosen to be observed 1 and 2 weeks after the irradiation.Blood cytokines were detected in their arterial blood.Any pathological changes of the esophagus were observed with HE staining under a light microscope at the same time. Results Irradiation-induced esophagitis was observed in the irradiation group 7 days after irradiation,with obvious exfoliation and necrosis of the esophagal epithelium mucosae.The submucosa were hyperaemic and dropsical with abundant inflammatory cell infiltration.The pathological changes of the esophagus were similar at 7 and 14 days after irradiation.However,the irradiation-induced esophagitis of rats in the amifostine group,the 90 min magnetic group and the 120 min magnetic group were relatively slighter and the blood leucocytes and neutrophis in those 3 groups were significantly lower than those in the irradiation group,while a tendency toward repair of the mucosa of the esophagus was detected.Serum TNF-α,IL-1 and IL-6 in the 90 min magnetic group and 120 min magnetic group were significantly lower than those in the irradiation group. Conclusions Both a rotating magnetic field and amifostine can help prevent and treat irradiation-induced esophagitis.Their therapeutic efficacy is similar.Exposure to a rotating magnetic field could inhibit the expression of inflammatory factors,and thus lessen the inflammatory reaction of acute irradiation-induced esophagitis.