CAJ | 학술논문

目的探讨喉癌组织傅立叶变换红外光谱(FTIR)特征及其分子水平变化,为FTIR诊断喉癌提供依据.方法应用FTIR仪检测32例喉癌患者的新鲜癌组织和癌旁正常组织,结合常规病理结果指认,总结二者的光谱特征.结果喉癌组织和癌旁正常组织的红外光谱表现出较大的差异性:(1)喉正常组织中1085 cm-1处核酸分子磷酸二脂基团VsP2-的对称伸缩振动谱带,在喉癌组织中向高波数方向位移,并且吸收强度增强;(2)喉癌组织中,1451 cm-1和1397 cm-1、2926 cm-1和2870 cm-1处吸收峰的相对峰强降低;(3)喉正常组织中的蛋白质酰胺谱带(1648 cm-1和1538cm-1)、蛋白质分子中甲基的对称和反对称弯曲振动谱带(1397 cm-1和1451 cm-1)、蛋白质分子C-OH伸缩振动谱带(1164 cm-1)和氢键化的NH基团谱带(3286 cm-1),在喉癌组织中均向高波数方向位移.与常规病理结果比较,仅1例癌旁正常组织被VHR指认为癌变,FHR检测准确率达98.4%.结论 FTIR可以反映喉癌组织和正常组织的蛋白质、核酸和脂类等生物大分子在含量、结构和构象上的差异,揭示喉癌组织的特征,有望成为喉癌术前早期诊断、术中辅助快速诊断的一种新方法.
목적 탐토후암조직부립협변환홍외광보(FTIR)특정급기분자수평변화,위FTIR진단후암제공의거.방법 응용FTIR의검측32례후암환자적신선암조직화암방정상조직,결합상규병리결과지인,총결이자적광보특정.결과 후암조직화암방정상조직적홍외광보표현출교대적차이성:(1)후정상조직중1085 cm-1처핵산분자린산이지기단VsP2-적대칭신축진동보대,재후암조직중향고파수방향위이,병차흡수강도증강;(2)후암조직중,1451 cm-1화1397 cm-1、2926 cm-1화2870 cm-1처흡수봉적상대봉강강저;(3)후정상조직중적단백질선알보대(1648 cm-1화1538cm-1)、단백질분자중갑기적대칭화반대칭만곡진동보대(1397 cm-1화1451 cm-1)、단백질분자C-OH신축진동보대(1164 cm-1)화경건화적NH기단보대(3286 cm-1),재후암조직중균향고파수방향위이.여상규병리결과비교,부1례암방정상조직피VHR지인위암변,FHR검측준학솔체98.4%.결론 FTIR가이반영후암조직화정상조직적단백질、핵산화지류등생물대분자재함량、결구화구상상적차이,게시후암조직적특정,유망성위후암술전조기진단、술중보조쾌속진단적일충신방법.
Objective To investigate the characteristics of freshly resected laryngeal carcinoma by Fourier transform infrared spectroscopy (FTIR). Methods FTIR was applied to the study of the cancerous tissues and adjacent normal tissues in 32 patients. Results Compared with pathological diagnosis results, one benign specimen was classified as a malignant, the accuracy was 98.4%. Significant differences were seen in the FTIR spectra between the normal and malignant laryngeal tissues. The peak at 1085 cm-1 shift to 1114 cm-1 showed that the relative contents of DNA in laryngeal carcinoma cells was increased. The peak at 1397cm-1 was stronger than 1451 cm-1 in normal tissues, while it was not obvious in cancer tissues. I2926/ I2870 in carcinoma cells was lower than that in normal tissues. The wave numbers of the bands of amide Ⅰ and amide Ⅱ, symmetric and asymmetric stretching bands of CH3, stretching vibration bands of C-OH and NH band were shifted to higher number in cancer tissues. Conclusion The study shows that the malignant and normal laryngeal tissues have different FTIR spectra, which are mainly due to changes in protein,nucleic acid and phospholipids. FTIR may become a new method for the diagnosis of laryngeal carcinoma in clinical practice.