CAJ | 학술논문

目的观察冷冻联合重组人肿瘤坏死因子-α( rhTN F-α)治疗C6大鼠脑胶质瘤治疗的协同作用.方法以原位末端转移酶标记(TUNEL)法检测肿瘤细胞的凋亡,采用免疫组织化学检测增殖细胞核抗原(PCNA)的表达；行核磁共振(MRI)检查,测量肿瘤体积,计算抑瘤率.动态观察各组荷瘤鼠生存期和rhTNF-α对荷瘤鼠的不良反应.结果联合治疗组肿瘤细胞凋亡、PCNA的表达、肿瘤体积和生存期与其他各组比较差异有统计学意义(P＜0.01),且该组对肿瘤生.长的抑制率(60.38％；89.48％)明显大于冷冻组(42.69％；66.31％)和TNF-α组(21.68％；49.01％),且大于理论抑瘤率(82.82％).rhTNF-α对实验动物有不良反应.结论冷冻和rhTNF-α对C6联用的抗肿瘤作用进一步加强,具有协同作用.
목적 관찰냉동연합중조인종류배사인자-α( rhTN F-α)치료C6대서뇌효질류치료적협동작용.방법 이원위말단전이매표기(TUNEL)법검측종류세포적조망,채용면역조직화학검측증식세포핵항원(PCNA)적표체；행핵자공진(MRI)검사,측량종류체적,계산억류솔.동태관찰각조하류서생존기화rhTNF-α대하류서적불량반응.결과 연합치료조종류세포조망、PCNA적표체、종류체적화생존기여기타각조비교차이유통계학의의(P＜0.01),차해조대종류생.장적억제솔(60.38％；89.48％)명현대우냉동조(42.69％；66.31％)화TNF-α조(21.68％；49.01％),차대우이론억류솔(82.82％).rhTNF-α대실험동물유불량반응.결론 냉동화rhTNF-α대C6련용적항종류작용진일보가강,구유협동작용.
Objective To observe the synergistic effects of freeze combination with recombinant human tumor necrosis factor-α (rhTNF-α) on the treatmemt of rat C6 brain glioma.Methods TdT-medi-ated dUTP nick end labeling (TUNEL) was applied to detect apoptosis.Proliferating cell nuclear antigen (PCNA) expression in tumor cell was examined by SP immunohistochemical staining.Meanwhile,the tumor volume were measured by MRI and tumor growth inhibition rate was acquired.The survival period of rats and the poison side effect of rhTNF-α were dynamically observed.Results After comparing the combined group with the other three groups,the statistical significance was exhibited about PCNA expression,tumor volume and survival period ( P ＜ 0.01 ).The inhibition rate of tumor growth in combined group (60.38％; 89.48％ )was significantly higher than in cryotherapy group (42.69％; 66.31％ )and in rhTNF-α group (21.68％ ; 49.01％ ),and even larger than tbeories inhibition rate ( 55.1 1％ ; 82.82％ ) of tumor growth.At the same time,the certain poison side effect to the experiment animal was caused by rhTNF-α.Conclusion The combination of freeze and rhTNF-α could strengthen efficiency of antitumor and had synergic effects in the treatment of glioma.