CAJ | 학술논문

目的评价肺水通道蛋白1(AQP1)表达在体外循环致犬肺损伤中的作用.方法健康杂种犬24只,体重15-20 kg,雌雄不拘,采用随机数字表法,将其随机分为4组(n=6):对照组(C组)、乙酰唑胺Ⅰ组(AⅠ组)、乙酰唑胺Ⅱ组(AⅡ组)和乙酰唑胺Ⅲ组(AⅢ组),开胸并制备犬CPB模型.C组采用传统预充液,AⅠ组、AⅡ组和AⅢ组分别在预充液中加入乙酰唑胺20、40、60 mg/kg.于开胸未行机械通气(T1)、停机(T2)和停机后1 h(T3)时取股动脉血,行动脉血气分析,计算呼吸指数 (RI)和氧合指数(OI),取肺组织采用RT-PCR及Westem-blot法检测AQP1 mRNA和蛋白表达,光镜下观察肺组织病理学结果并进行肺损伤评分.结果与C组比较,AⅠ组、AⅡ组和A Ⅲ组T2,3时P(A-a)O2和RI升高,OI降低,AQP1蛋白表达下调,肺损伤评分升高,AⅡ组和AⅢ组T2,3时AQPl mRNA表达下调(P＜0.05)；与AⅠ组组比较,AⅡ组和AⅢ组T2,3时P(A-a)O2和RI升高,OI降低,AQP1蛋白表达下调,肺损伤评分升高,AⅢ组T2,3时AQP1 mRNA表达下调(P＜0.05)；与AⅡ组比较,AⅢ组T2,3时RI升高,AQP1蛋白表达下调,肺损伤评分升高(P＜0.05).结论 AQP41表达下调参与了体外循环致犬肺损伤的过程.
목적 평개폐수통도단백1(AQP1)표체재체외순배치견폐손상중적작용.방법 건강잡충견24지,체중15-20 kg,자웅불구,채용수궤수자표법,장기수궤분위4조(n=6):대조조(C조)、을선서알Ⅰ조(AⅠ조)、을선서알Ⅱ조(AⅡ조)화을선서알Ⅲ조(AⅢ조),개흉병제비견CPB모형.C조채용전통예충액,AⅠ조、AⅡ조화AⅢ조분별재예충액중가입을선서알20、40、60 mg/kg.우개흉미행궤계통기(T1)、정궤(T2)화정궤후1 h(T3)시취고동맥혈,행동맥혈기분석,계산호흡지수 (RI)화양합지수(OI),취폐조직채용RT-PCR급Westem-blot법검측AQP1 mRNA화단백표체,광경하관찰폐조직병이학결과병진행폐손상평분.결과 여C조비교,AⅠ조、AⅡ조화A Ⅲ조T2,3시P(A-a)O2화RI승고,OI강저,AQP1단백표체하조,폐손상평분승고,AⅡ조화AⅢ조T2,3시AQPl mRNA표체하조(P＜0.05)；여AⅠ조조비교,AⅡ조화AⅢ조T2,3시P(A-a)O2화RI승고,OI강저,AQP1단백표체하조,폐손상평분승고,AⅢ조T2,3시AQP1 mRNA표체하조(P＜0.05)；여AⅡ조비교,AⅢ조T2,3시RI승고,AQP1단백표체하조,폐손상평분승고(P＜0.05).결론 AQP41표체하조삼여료체외순배치견폐손상적과정.
Objective To evaluate the role of aquaporin 1(AQPI)expression in the cartiopulmonary bypass(CPB)-induced lung injury in dogs.Methods Twenty-four healthy dogs,weighing 15-20 kg,were randomly divided into 4 group(n =6 each):control group(group C),acetazolamide Ⅰ group(group A Ⅰ),acetazolamide Ⅱ group(group A Ⅱ),and acetazolarnide Ⅲ group(group AⅢ).Lung injury was produced by CPB.The traditional priming solution was infused in group C.Priming solutions containing acetaaolamide 20,40 and 60 mg/kgwere infused in groups A Ⅰ,A Ⅱ and A Ⅲ respectively.Blood samples were collected from the femoral artery before mechanical ventilation,at the end of CPB and at 1 h after end of CPB(T1-3)for arterial blood gas analysis.Respiration index(RI)and oxygenation index(OI)were calculated.The lung specimens were oblained for determination of AQPI mRNA and protein expression(by RT-PCR and Western blot)and for microscopic examination.The pathological changes of the lung were scored.Results Compared with group C,P(A-a)O2,RI and the pathological score were significantly increased at T2.3,OI was significantly decreased at T2.3,and AQP1 protein expression was down-regulated at T2.3 in groups A Ⅰ,A Ⅱ and AⅢ,and AQP1 mRNA expression was down-regulated at T2.3 in groups AⅡ and AⅢ(P＜0.05).Compared with group A Ⅰ,P(A-a)O2,RI and the pathological score were significantly increased at T2.3,OI was significantly decreased at T2.3,and AQP1 protein expression was down-regulated at T2.3 in groups A Ⅱ and AⅢ,and AQP1 mRNA expression was down-regulated at T2.3 ingroup A Ⅲ(P ＜ 0.05).Compared with group A Ⅱ,RI and the pathological score were significantly increased at T2.3,and AQP1 protein expression was down-regulated at T2.3 in group A Ⅲ(P ＜ 0.05).Conclutsion Down-regulation of AQPI expression is involved in the CPB-induced lung injury in dogs.