CAJ | 학술논문

目的观察细胞骨架分子Fascin-1,Ezrin及Paxillin在喉鳞状细胞癌(简称鳞癌)组织中的表达,并评价三者对喉鳞癌复发风险及预后的预测价值.方法采用免疫组化Max Vision二步法观察三种蛋白在199例患者喉鳞癌组织中的表达情况,通过非条件Logistic回归及单因素、多因素生存分析,总结三者在喉鳞癌复发风险预测及预后评估中的价值.结果三种蛋白分别在低分化、颈淋巴转移及临床晚期(Ⅲ+Ⅳ)喉鳞癌组织中表达增强趋势(P值均＜0.05),且三者表达呈正相关趋势(P值均＜0.001).三种蛋白分别在复发组中的表达强度高于未复发组(x2值分别为42.479,43.673和22.261,P＜0.05).三种蛋白联合高表达患者中复发病例构成比为69.1％,高于其他联合表达模式组中的构成比(P ＜0.001),且Fascin-1(比值比为7.89,95％可信区间为2.26～27.53,P=0.001)及Ezrin(比值比为2.51,95％可信区间为1.18 ～5.32,P＜0.001)为影响喉鳞癌复发的独立危险因素.三种蛋白分别高表达患者的5年无瘤生存率均降低(P＜0.05),而三者联合高表达患者5年无瘤生存率更低,为26.4％ (P＜0.05).三种蛋白均为影响患者预后独立危险因素(P＜0.05).结论 Fascin-1,Ezrin,Paxillin不仅与喉鳞癌的恶性进展相关,还与复发风险及预后不良相关.联合检测三者表达情况,有助于喉鳞癌复发风险预测及预后评估.
목적 관찰세포골가분자Fascin-1,Ezrin급Paxillin재후린상세포암(간칭린암)조직중적표체,병평개삼자대후린암복발풍험급예후적예측개치.방법 채용면역조화Max Vision이보법관찰삼충단백재199례환자후린암조직중적표체정황,통과비조건Logistic회귀급단인소、다인소생존분석,총결삼자재후린암복발풍험예측급예후평고중적개치.결과 삼충단백분별재저분화、경림파전이급림상만기(Ⅲ+Ⅳ)후린암조직중표체증강추세(P치균＜0.05),차삼자표체정정상관추세(P치균＜0.001).삼충단백분별재복발조중적표체강도고우미복발조(x2치분별위42.479,43.673화22.261,P＜0.05).삼충단백연합고표체환자중복발병례구성비위69.1％,고우기타연합표체모식조중적구성비(P ＜0.001),차Fascin-1(비치비위7.89,95％가신구간위2.26～27.53,P=0.001)급Ezrin(비치비위2.51,95％가신구간위1.18 ～5.32,P＜0.001)위영향후린암복발적독립위험인소.삼충단백분별고표체환자적5년무류생존솔균강저(P＜0.05),이삼자연합고표체환자5년무류생존솔경저,위26.4％ (P＜0.05).삼충단백균위영향환자예후독립위험인소(P＜0.05).결론 Fascin-1,Ezrin,Paxillin불부여후린암적악성진전상관,환여복발풍험급예후불량상관.연합검측삼자표체정황,유조우후린암복발풍험예측급예후평고.
Objective To study the expressions of key assemblies of cytoskeleton,Fascin-1,Ezrin and Paxillin,in laryngeal squamous cell carcinoma (LSCC) and their correlation with clinicopathologic characteristics,cancer recurrence and survival of patients with LSCC. Methods The expressions of Fascin-1,Ezrin and Paxillin proteins were detected by immunohistochemistry in 199 cases of LSCC.Unconditional Logistic regression model or Cox proportional hazards model was used for the analyses of recurrent risks and prognostic factors. Results Significantly increased expression of Fascin-1,Ezrin or Paxillin expression was showed in the LSCC with poorly differentiated,positively cervical lymph nodal metastasis,and clinical stage Ⅲ+ Ⅳ respectively ( P ＜ 0.05 ).The expressions of three kinds of proteins in the recurrent cases were higher than those in non-recurrent cases respectively ( x2 were 42.479,43.673 and 22.261,P ＜0.05).The highest recurrence rate(69.1％ ) was observed in group of cases with the highly co-expression of the three kinds of proteins( P ＜ 0.05 ).The expression of Fascin-1 ( OR =7.89,95％ CI 2.26 - 27.53,P =0.001 ),or Ezrin ( OR =2.51,95％ CI 1.18 - 5.32,P ＜ 0.001 ) was independent risk for recurrence.Five-year disease-free survival rates of patients with high expression of Fascin-1,Ezrin or Paxillin were lower than those of patients with negative or low expressions for the proteins ( P ＜ 0.05 ).Patients with highly co-expression of three kinds of proteins showed the poorest survival prognosis,with a 5-year disease free survival ( DFS ) of only 26.4％ ( P ＜ 0.05 ),and expressions of three proteins were independent prognostic factors for 5-year DFS ( P ＜ 0.05 ).Conclusion Fascin-1,Ezrin,and Paxillin were correlative with LSCC progression and might be potential predictors for cancer recurrence and survival of patients with LSCC,as well as therapeutic targets for LSCC.