中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
10期
887-889
,共3页
王怀颖%石少慧%史建红%张悦
王懷穎%石少慧%史建紅%張悅
왕부영%석소혜%사건홍%장열
银杏叶提取物%环腺苷酸反应元件结合蛋白%衰老%大鼠%皮层
銀杏葉提取物%環腺苷痠反應元件結閤蛋白%衰老%大鼠%皮層
은행협제취물%배선감산반응원건결합단백%쇠로%대서%피층
Extract of Ginkgo biloba%cAMP response element binding protein%Aging%Rat%Cortex
目的 研究银杏叶片对衰老大鼠皮层核转录因子cAMP反应元件结合蛋白(CREB)、磷酸化CREB(pCREB)表达的影响.方法 雄性Wistar大鼠分为青年对照组、老年对照组和银杏叶组.银杏叶组大鼠每日灌胃银杏叶片,其余两组动物灌胃等量蒸馏水,共8周.Morris水迷宫实验观察大鼠空间记忆变化,western blot检测皮层CREB、pCREB表达.结果 (1)与青年对照组(9.6±2.88,41.55±6.30)比较,老年大鼠逃避潜伏期延长,穿越平台次数(6.8±2.49)、平台象限停留时间(34.92±4.56)减少(P＜0.05)；与老年对照组比较,EGb组大鼠逃避潜伏期缩短,穿越平台次数和平台象限停留时间(9.4±2.63,41.0±6.68)延长(P＜0.05).(2)老年对照组大鼠皮层CREB、pCREB表达水平分别为(0.70±0.21,0.13±0.05),低于青年对照组(1.07 ±0.33,0.26±0.04),差异具有显著性(P＜0.05)；与老年对照组比较,银杏叶组大鼠皮层CREB、pCREB表达升高(1.02±0.18,0.18±0.02) (P＜O.05).结论 银杏叶片可能通过上调老年大鼠大脑皮层CREB、pCREB表达改善空间学习和记忆功能.
目的 研究銀杏葉片對衰老大鼠皮層覈轉錄因子cAMP反應元件結閤蛋白(CREB)、燐痠化CREB(pCREB)錶達的影響.方法 雄性Wistar大鼠分為青年對照組、老年對照組和銀杏葉組.銀杏葉組大鼠每日灌胃銀杏葉片,其餘兩組動物灌胃等量蒸餾水,共8週.Morris水迷宮實驗觀察大鼠空間記憶變化,western blot檢測皮層CREB、pCREB錶達.結果 (1)與青年對照組(9.6±2.88,41.55±6.30)比較,老年大鼠逃避潛伏期延長,穿越平檯次數(6.8±2.49)、平檯象限停留時間(34.92±4.56)減少(P＜0.05)；與老年對照組比較,EGb組大鼠逃避潛伏期縮短,穿越平檯次數和平檯象限停留時間(9.4±2.63,41.0±6.68)延長(P＜0.05).(2)老年對照組大鼠皮層CREB、pCREB錶達水平分彆為(0.70±0.21,0.13±0.05),低于青年對照組(1.07 ±0.33,0.26±0.04),差異具有顯著性(P＜0.05)；與老年對照組比較,銀杏葉組大鼠皮層CREB、pCREB錶達升高(1.02±0.18,0.18±0.02) (P＜O.05).結論 銀杏葉片可能通過上調老年大鼠大腦皮層CREB、pCREB錶達改善空間學習和記憶功能.
목적 연구은행협편대쇠로대서피층핵전록인자cAMP반응원건결합단백(CREB)、린산화CREB(pCREB)표체적영향.방법 웅성Wistar대서분위청년대조조、노년대조조화은행협조.은행협조대서매일관위은행협편,기여량조동물관위등량증류수,공8주.Morris수미궁실험관찰대서공간기억변화,western blot검측피층CREB、pCREB표체.결과 (1)여청년대조조(9.6±2.88,41.55±6.30)비교,노년대서도피잠복기연장,천월평태차수(6.8±2.49)、평태상한정류시간(34.92±4.56)감소(P＜0.05)；여노년대조조비교,EGb조대서도피잠복기축단,천월평태차수화평태상한정류시간(9.4±2.63,41.0±6.68)연장(P＜0.05).(2)노년대조조대서피층CREB、pCREB표체수평분별위(0.70±0.21,0.13±0.05),저우청년대조조(1.07 ±0.33,0.26±0.04),차이구유현저성(P＜0.05)；여노년대조조비교,은행협조대서피층CREB、pCREB표체승고(1.02±0.18,0.18±0.02) (P＜O.05).결론 은행협편가능통과상조노년대서대뇌피층CREB、pCREB표체개선공간학습화기억공능.
Objective To investigate effects of Ginkgo biloba extract(EGb) on expression of CREB and pCREB in cortex of aging rats.Methods Male Wistar rats were divided into three groups:young control group,old control group and EGb group.Rats in EGb group were treated with intragastric administration of EGb,while rats in the other two groups were treated with distilled water.The spatial learning and memory were evaluated by Morris water maze,and the expression of CREB,pCREB were detected by western blot.Results ( 1 ) Compared with young control group (9.6 ± 2.88,41.55 ± 6.30),the swimming time and times through platform in the target quardrant of rats in old control group(6.8 ± 2.49,34.92 ± 4.56) were reduced (P ＜ 0.05 ).The times passing through the platform and the time exploring the target quadrant were more and longer in EGb group(9.4 ± 2.63,41.0 ± 6.68 ) than those in old control group(P ＜ 0.05 ).(2)Compared with rats in young control group( 1.07 ±0.33,0.26 ± 0.04),relative contents of CREB and p-CREB proteins in cortex (0.70 ± 0.21,0.13 ± 0.05 ) weredecreased in old control group(P＜0.05 ).CREB and p-CREB Levels were higher in EGb group ( 1.02 ±0.18,0.18 ±0.02)than those in old control group(P ＜ 0.05 ).Conclusion EGb can ameliorate spatial learning and memory of rats by increasing the expression of CREB and p-CREB in cortex.
