国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2009年
6期
521-524
,共4页
张邓新%张焰%尤杰%孙大鹏
張鄧新%張燄%尤傑%孫大鵬
장산신%장염%우걸%손대붕
七氟烷%后处理%胰岛素样生长因子-1%再灌注损伤
七氟烷%後處理%胰島素樣生長因子-1%再灌註損傷
칠불완%후처리%이도소양생장인자-1%재관주손상
Sevoflurane%Postconditioning%Insulin -like growth factor-1%Ischemia-reperfusion injury
目的 观察不同浓度七氟烷后处理对大鼠海马缺血性损伤的保护作用及其对胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)表达的影响.方法 选取雄性SD大鼠,随机分为6组:假手术组(S)、缺血但无后处理组(C)、吸氧后处理组(O_2)、不同浓度七氟烷后处理组(Sevo1组、Sevo2组、Sevo3组);不同浓度七氟烷后处理组于脑缺血/再灌注即刻给予吸入1.5%(Sevo1组)、2.4%(Sevo2组)、3.0%(Sevo3组)的七氟烷30 min.采用大脑中动脉闭塞(MCAO)改良模型,于再灌注后的24、48、72h对海马进行组织病理学观察及IGF-1 mRNA检测.结果 与S组相比,C组各时间点大鼠海马IGF-1 mRNA的表达增加(P＜0.05)且海马组织出现病理学损伤;与C组相比,Scvo2组、Scvo3组各时间点的海马IGF-1 mRNA表达进一步增加(P＜0.05),且海马组织病理学损伤较轻;Sevo1组与C组相比,海马IGF-1mRNA的表达及病理学损伤无明显差异.结论 较高浓度的七氟烷后处理可以通过上调海马IGF-1及其受体系统而发挥中枢神经保护作用.
目的 觀察不同濃度七氟烷後處理對大鼠海馬缺血性損傷的保護作用及其對胰島素樣生長因子-1(insulin-like growth factor-1,IGF-1)錶達的影響.方法 選取雄性SD大鼠,隨機分為6組:假手術組(S)、缺血但無後處理組(C)、吸氧後處理組(O_2)、不同濃度七氟烷後處理組(Sevo1組、Sevo2組、Sevo3組);不同濃度七氟烷後處理組于腦缺血/再灌註即刻給予吸入1.5%(Sevo1組)、2.4%(Sevo2組)、3.0%(Sevo3組)的七氟烷30 min.採用大腦中動脈閉塞(MCAO)改良模型,于再灌註後的24、48、72h對海馬進行組織病理學觀察及IGF-1 mRNA檢測.結果 與S組相比,C組各時間點大鼠海馬IGF-1 mRNA的錶達增加(P＜0.05)且海馬組織齣現病理學損傷;與C組相比,Scvo2組、Scvo3組各時間點的海馬IGF-1 mRNA錶達進一步增加(P＜0.05),且海馬組織病理學損傷較輕;Sevo1組與C組相比,海馬IGF-1mRNA的錶達及病理學損傷無明顯差異.結論 較高濃度的七氟烷後處理可以通過上調海馬IGF-1及其受體繫統而髮揮中樞神經保護作用.
목적 관찰불동농도칠불완후처리대대서해마결혈성손상적보호작용급기대이도소양생장인자-1(insulin-like growth factor-1,IGF-1)표체적영향.방법 선취웅성SD대서,수궤분위6조:가수술조(S)、결혈단무후처리조(C)、흡양후처리조(O_2)、불동농도칠불완후처리조(Sevo1조、Sevo2조、Sevo3조);불동농도칠불완후처리조우뇌결혈/재관주즉각급여흡입1.5%(Sevo1조)、2.4%(Sevo2조)、3.0%(Sevo3조)적칠불완30 min.채용대뇌중동맥폐새(MCAO)개량모형,우재관주후적24、48、72h대해마진행조직병이학관찰급IGF-1 mRNA검측.결과 여S조상비,C조각시간점대서해마IGF-1 mRNA적표체증가(P＜0.05)차해마조직출현병이학손상;여C조상비,Scvo2조、Scvo3조각시간점적해마IGF-1 mRNA표체진일보증가(P＜0.05),차해마조직병이학손상교경;Sevo1조여C조상비,해마IGF-1mRNA적표체급병이학손상무명현차이.결론 교고농도적칠불완후처리가이통과상조해마IGF-1급기수체계통이발휘중추신경보호작용.
Objective To observe effects of sevoflurane postconditioning against ischemia injury and on the expression of insulin-like growth factor-1 in rat hippocampus with different inhalation doses. Methods Experiments were performed using male SD rats which were randomly assigned to 6 groups: sham operation group (group S); ischemia-reperfusion group (group C); oxygen postconditioning group (group O_2); sevoflurane postconditioning group with inhaled concentration of 1.5% (group Sevol), 2.4% (group Sevo2) or 3.0% (group Sevo3). All animals were subjected to the modified model of right middle cerebral artery occlusion (MCAO) for 120 min. The histopathology of hippocampal slices and the IGF-1 mRNA expression of hippocampus were recorded at 24, 48, and 72h after reperfusion. Results In group C, IGF-1 mRNA expression increased and the obvious histopathologic damage in hippocampus was observed. The IGF -1 mRNA expression further increased and the histopathologic damage in hippocampus attenuated in groups Sevo2 and Sevo3, but not in group Sevol, compared to that in group C. Conclusion Sevoflurane postconditioning with higher inhalation dose exert neuroprotective effects against ischemia injury via up-regulating IGF-1 receptor system in rat hippocampus.
