CAJ | 학술논문

目的探讨辐射损伤后大鼠鼻黏膜早期组织重塑的特点.方法清洁级SD大鼠40只,雄性,采用随机数字排列法将动物随机分组,对照组、20 Gy组、30 Gy组、4JD Gy组和50 Gy组,每组8只.辐射完成后2周,取出中鼻甲进行组织学处理:采用苏木素-伊红(hematoxylin-eosin,HE)染色,计数中鼻甲黏膜内嗜酸粒细胞数并测定上皮细胞损伤程度;采用阿辛蓝-过碘酸.希夫(alcian blue-periodic acid-Sehff,AB-PAS)染色,计数中鼻甲黏膜中单位面积上皮细胞中杯状细胞数;采用Masson三色胶原染色Masson trichmme(MT)法染色,测定中鼻甲黏膜内胶原成分染色的百分比面积.结果对照组少量嗜酸粒细胞浸润,实验组均有大量嗜酸粒细胞浸润,并以30 Gy组嗜酸性粒细胞浸润最多.20 Gy组未见明显的上皮细胞损伤,其他各组均见不同程度的上皮细胞损伤,吸收剂量越大,上皮细胞损伤程度越重.20 Gy组杯状细胞数略增多,但差异无统计学意义;30 Gy组和40 Gy组杯状细胞明显增多,50 Gy组杯状细胞数明显减少.细胞外基质沉积在20 Gy组略增多,但差异无统计学意义;其他各组明显增多,且随着剂量的增大,MT染色蓝色所占的百分比面积有增多的趋势.结论黏膜上皮细胞损伤、嗜酸性粒细胞浸润、杯状细胞化生和黏膜内胶原成分增加是早期放射性鼻黏膜损伤后黏膜上皮组织重塑的特点.组织重塑随着辐射剂量和辐射时间呈现一定的变化规律.
목적 탐토복사손상후대서비점막조기조직중소적특점.방법 청길급SD대서40지,웅성,채용수궤수자배렬법장동물수궤분조,대조조、20 Gy조、30 Gy조、4JD Gy조화50 Gy조,매조8지.복사완성후2주,취출중비갑진행조직학처리:채용소목소-이홍(hematoxylin-eosin,HE)염색,계수중비갑점막내기산립세포수병측정상피세포손상정도;채용아신람-과전산.희부(alcian blue-periodic acid-Sehff,AB-PAS)염색,계수중비갑점막중단위면적상피세포중배상세포수;채용Masson삼색효원염색Masson trichmme(MT)법염색,측정중비갑점막내효원성분염색적백분비면적.결과 대조조소량기산립세포침윤,실험조균유대량기산립세포침윤,병이30 Gy조기산성립세포침윤최다.20 Gy조미견명현적상피세포손상,기타각조균견불동정도적상피세포손상,흡수제량월대,상피세포손상정도월중.20 Gy조배상세포수략증다,단차이무통계학의의;30 Gy조화40 Gy조배상세포명현증다,50 Gy조배상세포수명현감소.세포외기질침적재20 Gy조략증다,단차이무통계학의의;기타각조명현증다,차수착제량적증대,MT염색람색소점적백분비면적유증다적추세.결론 점막상피세포손상、기산성립세포침윤、배상세포화생화점막내효원성분증가시조기방사성비점막손상후점막상피조직중소적특점.조직중소수착복사제량화복사시간정현일정적변화규률.
Objective To explore the feature of nasal mucosa remodeling in experimental radiation injury. Methods Fourty male rats were randomly divided into five groups, as control group and radiation injury groups (radiation dose were 20 Gy,30 Gy,40 Gy and 50 Gy). Each group had 8 rats. Two weeks after the last irradiation, the rats were killed and the nasal middle turbinates of the animals were removed. The tissue blocks were embedded in paraffin. The paraffin sections were stained with hematoxylin and eosin (HE), alcian blue-periodic acid-Schif ( AB-PAS), and Massen Trichreme ( MT). The infiltrating eosinophils in nasal mucosa were examined. AB-PAS positive cells in the surface epithelium in nasal mucosa were counted. The percentage of area in MT stained extracellular matrix in nasal mucosa and damage of epithelium were determined by an image analyzer. Results The control group only presented a few eosinophils. Significant eosinophil infiltration was observed in the radiation injury groups, especially for the 30 Gy radiation injury group. Compared with the control group, there was no significant epithelial damage in 20 Gy radiation injury group. Significant epithelial damage were observed in the rest of radiation injury groups. The epithelial damage became more severe as the radiation dose increasing. A little but not significant increase in AB-PAS positive cells was observed in the mucos of the 20 Gy radiation injury group and significant increase in the 30 and 40 Gy groups. But in the 50 Gy radiation injury group, the AB-PAS positive cells were decreased compared with control group. The collagen fibrils in the mucosa of nasal middle turbinate in 20 Gy radiation injury group did not significantly increase. But in the other groups, the increase was significant compared with that of control group. Furthermore, collagen fibrils increased as the radiation dose increased. Conclusions Epithelial damage, goblet cells hyperplasia and extracellular matrix deposition are the features of remodeling in the rats model of early radiation injury.