中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
5期
270-275
,共6页
区雪婷%吴吉芹%朱利平%章强强%王菲菲%徐斌%胡秀平%王璇%王睿莹%翁心华
區雪婷%吳吉芹%硃利平%章彊彊%王菲菲%徐斌%鬍秀平%王璇%王睿瑩%翁心華
구설정%오길근%주리평%장강강%왕비비%서빈%호수평%왕선%왕예형%옹심화
甘露糖%凝集素类%隐球菌病%脑膜炎,隐球菌性%多态现象,遗传%疾病遗传易感性
甘露糖%凝集素類%隱毬菌病%腦膜炎,隱毬菌性%多態現象,遺傳%疾病遺傳易感性
감로당%응집소류%은구균병%뇌막염,은구균성%다태현상,유전%질병유전역감성
Mannose%Agglutinins%Cryptococcosis%Meningitis,cryptococcal%Polymorphism,genetic%Genetic predisposition to disease
目的 分析我国非AIDS隐球菌病患者甘露糖结合凝集素(MBL)基因多态性分布情况,并探讨其与隐球菌病易感性的关系.方法 采用病例一对照遗传关联研究,病例组为非AIDS相关隐球菌病患者167例,包括隐球菌脑膜炎103例和肺隐球菌感染64例,健康对照组为体检者208例.提取受试者外周血标本基因组DNA,PCR特异性扩增MBL基因DNA片段,对扩增产物进行序列分析,以检测MBL单核苷酸多态性(SNP),进一步探讨MBL基因多态性与隐球菌病易感性的遗传关联性.病例组与对照组比较采用χ2检验或Fisher确切概率检验,MBL基因型各组血浆MBL差异比较采用单因素方差分析.结果 167例隐球菌病患者MBL各等位基因、单倍型、基因型所占比例与健康对照组比较,差异均无统计学意义(P>0.05).但是,隐球菌脑膜炎患者MBL基因型缺陷组的构成比(16.5%)显著高于健康对照组(8.7%,χ2=4.25,P=0.0392,OR=2.09),在免疫正常隐球菌脑膜炎患者中(21.4%)差异更为显著(χ2=7.15,P=0.0075,OR=2.88).此外,隐球菌脑膜炎患者MBL基因型缺陷组的构成比显著高于非中枢神经系统感染患者(3.1%,Fisher确切概率检验,P=0.010,OR=6.13),在免疫正常患者中(分别为21.4%和4.0%)差异更为显著(P=0.009,OR=6.55).结论 MBL基因型缺陷是隐球菌脑膜炎的遗传易感因素,MBL缺陷的隐球菌病患者中枢神经系统更易受累.
目的 分析我國非AIDS隱毬菌病患者甘露糖結閤凝集素(MBL)基因多態性分佈情況,併探討其與隱毬菌病易感性的關繫.方法 採用病例一對照遺傳關聯研究,病例組為非AIDS相關隱毬菌病患者167例,包括隱毬菌腦膜炎103例和肺隱毬菌感染64例,健康對照組為體檢者208例.提取受試者外週血標本基因組DNA,PCR特異性擴增MBL基因DNA片段,對擴增產物進行序列分析,以檢測MBL單覈苷痠多態性(SNP),進一步探討MBL基因多態性與隱毬菌病易感性的遺傳關聯性.病例組與對照組比較採用χ2檢驗或Fisher確切概率檢驗,MBL基因型各組血漿MBL差異比較採用單因素方差分析.結果 167例隱毬菌病患者MBL各等位基因、單倍型、基因型所佔比例與健康對照組比較,差異均無統計學意義(P>0.05).但是,隱毬菌腦膜炎患者MBL基因型缺陷組的構成比(16.5%)顯著高于健康對照組(8.7%,χ2=4.25,P=0.0392,OR=2.09),在免疫正常隱毬菌腦膜炎患者中(21.4%)差異更為顯著(χ2=7.15,P=0.0075,OR=2.88).此外,隱毬菌腦膜炎患者MBL基因型缺陷組的構成比顯著高于非中樞神經繫統感染患者(3.1%,Fisher確切概率檢驗,P=0.010,OR=6.13),在免疫正常患者中(分彆為21.4%和4.0%)差異更為顯著(P=0.009,OR=6.55).結論 MBL基因型缺陷是隱毬菌腦膜炎的遺傳易感因素,MBL缺陷的隱毬菌病患者中樞神經繫統更易受纍.
목적 분석아국비AIDS은구균병환자감로당결합응집소(MBL)기인다태성분포정황,병탐토기여은구균병역감성적관계.방법 채용병례일대조유전관련연구,병례조위비AIDS상관은구균병환자167례,포괄은구균뇌막염103례화폐은구균감염64례,건강대조조위체검자208례.제취수시자외주혈표본기인조DNA,PCR특이성확증MBL기인DNA편단,대확증산물진행서렬분석,이검측MBL단핵감산다태성(SNP),진일보탐토MBL기인다태성여은구균병역감성적유전관련성.병례조여대조조비교채용χ2검험혹Fisher학절개솔검험,MBL기인형각조혈장MBL차이비교채용단인소방차분석.결과 167례은구균병환자MBL각등위기인、단배형、기인형소점비례여건강대조조비교,차이균무통계학의의(P>0.05).단시,은구균뇌막염환자MBL기인형결함조적구성비(16.5%)현저고우건강대조조(8.7%,χ2=4.25,P=0.0392,OR=2.09),재면역정상은구균뇌막염환자중(21.4%)차이경위현저(χ2=7.15,P=0.0075,OR=2.88).차외,은구균뇌막염환자MBL기인형결함조적구성비현저고우비중추신경계통감염환자(3.1%,Fisher학절개솔검험,P=0.010,OR=6.13),재면역정상환자중(분별위21.4%화4.0%)차이경위현저(P=0.009,OR=6.55).결론 MBL기인형결함시은구균뇌막염적유전역감인소,MBL결함적은구균병환자중추신경계통경역수루.
Objective To describe the distribution of mannose binding lectin (MBL) genetic polymorphisms in non-acquired immunodeficiency syndrome (AIDS) patients with cryptococcosis in China and to verify the association of MBL polymorphisms with susceptibility to cryptococcosis.Methods The case-controlled genetic association study was conducted and 167 non-AIDS patients with cryptococcosis and 208 healthy controls were recruited. Genome DNA was extracted from the peripheral blood and MBL gene was amplified by polymerase chain reaction (PCR). Six singlenucleotide polymorphisms ( SNP) of MBL gene were sequenced. The association of MBL polymorphisms with susceptibility to cryptococcosis were analyzed. The comparison between patients and controls was performed by chi square test or Fisher's exact test. The differences of MBL plasma concentrations between groups with different MBL genotypes were compared by single factor variance analysis. Results There were no differences between patients and controls in terms of MBL genotype frequencies, haplotypes and genotypes (all P>0. 05). Compared with healthy control, the deficient MBL-producing genotypes were strongly associated with cryptococcal meningitis (16. 5% vs 8. 7%,χ2=4.25, P=0.0392, OR = 2.09), particularly in patients without underlying immunocompromised conditions (21. 4% vs 8. 7%, χ2 =7. 15, P = 0. 0075, OR = 2. 88). Individuals with MBL deficiency genotypes showed significantly higher rates of central nervous system (CNS) cryptococcal infection rather than non-CNS cryptococcosis (16. 5% vs 3. 1%, Fisher's exact test, P = 0. 010, OR = 6. 13).The difference was even more significant in the immunocompetent patients (21. 4% vs 4. 0%, P =0.009, OR= 6. 55). Conclusion MBL deficiency is associated with cryptococcal meningitis and may play a role in CNS Cryptococcus infection.
