中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2010年
4期
225-229
,共5页
黄娟%代琳%雷松%廖殿英%王晓卿%罗添友%陈昱%杭振镳%李甘地%董丹丹%徐纲%辜正策%郝冀玲%华平%何磊%段芳蕾
黃娟%代琳%雷鬆%廖殿英%王曉卿%囉添友%陳昱%杭振鑣%李甘地%董丹丹%徐綱%辜正策%郝冀玲%華平%何磊%段芳蕾
황연%대림%뢰송%료전영%왕효경%라첨우%진욱%항진표%리감지%동단단%서강%고정책%학기령%화평%하뢰%단방뢰
猫抓病%巴尔通体感染%银染色法%免疫组织化学%显微镜检查,电子
貓抓病%巴爾通體感染%銀染色法%免疫組織化學%顯微鏡檢查,電子
묘조병%파이통체감염%은염색법%면역조직화학%현미경검사,전자
Cat-scratch disease%Bartonella infections%Silver staining%Immunohiatochemistry%Microscopy,electron
目的 探讨Warthin-Starry银染色法、抗汉赛巴尔通体单克隆抗体和电镜在检测人巴尔通体感染、确诊猫抓病中的实用价值.方法 收集1998年1月至2008年12月诊断的77例符合猫抓病形态学改变的石蜡包埋淋巴结组织,分别使用Warthin-Starry银染色法和抗汉赛巴尔通体单克隆抗体的EnVision法进行病原体的检测.选择二者均检出丰富细菌的5例进行透射电镜观察.结果 (1)透射电镜查见汉赛巴尔通体呈多形性,圆形、椭圆形、短棒状、杆状,长0.489～1.110μm,宽0.333～0.534 tun,集群性生长.(2)61.0%(47/77)的病例Warthin-Starry银染色法查见排列成线、成簇、局灶性聚集的黑色、短小、棒状杆菌,细菌绝大多数分布在靠近被膜下的坏死灶内,以存在于细胞外为主.(3)72.7%(56/77)的病例抗汉赛巴尔通体单克隆抗体阳性表达,阳性信号主要呈点状、颗粒状,少数呈线样勾勒出细菌形状,信号定位与Warthin-Starry银染色法一致.(4)59.7%(46/77)的病例Warthin-Starry银染色和抗汉赛巴尔通体单克隆抗体染色均阳性.两种方法联用,病原体总体检出率为74.0%(57/77).二种方法检出率的差异具有统计学意义(P＜0.05).结论 应用Warthin-Starry银染色法、免疫组织化学和电镜技术检测猫抓病病原体能达到确诊猫抓病的目的 ,抗汉赛巴尔通体单克隆抗体染色是较Warthin-Starry银染色更理想的检测方法.
目的 探討Warthin-Starry銀染色法、抗漢賽巴爾通體單剋隆抗體和電鏡在檢測人巴爾通體感染、確診貓抓病中的實用價值.方法 收集1998年1月至2008年12月診斷的77例符閤貓抓病形態學改變的石蠟包埋淋巴結組織,分彆使用Warthin-Starry銀染色法和抗漢賽巴爾通體單剋隆抗體的EnVision法進行病原體的檢測.選擇二者均檢齣豐富細菌的5例進行透射電鏡觀察.結果 (1)透射電鏡查見漢賽巴爾通體呈多形性,圓形、橢圓形、短棒狀、桿狀,長0.489～1.110μm,寬0.333～0.534 tun,集群性生長.(2)61.0%(47/77)的病例Warthin-Starry銀染色法查見排列成線、成簇、跼竈性聚集的黑色、短小、棒狀桿菌,細菌絕大多數分佈在靠近被膜下的壞死竈內,以存在于細胞外為主.(3)72.7%(56/77)的病例抗漢賽巴爾通體單剋隆抗體暘性錶達,暘性信號主要呈點狀、顆粒狀,少數呈線樣勾勒齣細菌形狀,信號定位與Warthin-Starry銀染色法一緻.(4)59.7%(46/77)的病例Warthin-Starry銀染色和抗漢賽巴爾通體單剋隆抗體染色均暘性.兩種方法聯用,病原體總體檢齣率為74.0%(57/77).二種方法檢齣率的差異具有統計學意義(P＜0.05).結論 應用Warthin-Starry銀染色法、免疫組織化學和電鏡技術檢測貓抓病病原體能達到確診貓抓病的目的 ,抗漢賽巴爾通體單剋隆抗體染色是較Warthin-Starry銀染色更理想的檢測方法.
목적 탐토Warthin-Starry은염색법、항한새파이통체단극륭항체화전경재검측인파이통체감염、학진묘조병중적실용개치.방법 수집1998년1월지2008년12월진단적77례부합묘조병형태학개변적석사포매림파결조직,분별사용Warthin-Starry은염색법화항한새파이통체단극륭항체적EnVision법진행병원체적검측.선택이자균검출봉부세균적5례진행투사전경관찰.결과 (1)투사전경사견한새파이통체정다형성,원형、타원형、단봉상、간상,장0.489～1.110μm,관0.333～0.534 tun,집군성생장.(2)61.0%(47/77)적병례Warthin-Starry은염색법사견배렬성선、성족、국조성취집적흑색、단소、봉상간균,세균절대다수분포재고근피막하적배사조내,이존재우세포외위주.(3)72.7%(56/77)적병례항한새파이통체단극륭항체양성표체,양성신호주요정점상、과립상,소수정선양구륵출세균형상,신호정위여Warthin-Starry은염색법일치.(4)59.7%(46/77)적병례Warthin-Starry은염색화항한새파이통체단극륭항체염색균양성.량충방법련용,병원체총체검출솔위74.0%(57/77).이충방법검출솔적차이구유통계학의의(P＜0.05).결론 응용Warthin-Starry은염색법、면역조직화학화전경기술검측묘조병병원체능체도학진묘조병적목적 ,항한새파이통체단극륭항체염색시교Warthin-Starry은염색경이상적검측방법.
Objective To evaluate the diagnostic utility of Warthin-Starry silver stain,immunohiatochemistry and transmission electron microscopy in the detection of human Bartonella henselae infection and pathologic diagnosis of cat scratch disease(CSD).Metbotis The paraffin-embedded lymph node tissues of 77 histologically.defined cases of cat scratch disease collected during the period from January,1998 to December,2008 were retrieved and studied using Warthin-Starry silver stain(WS stain)and mouse monoclonal antibody against Bartonella henselae(BhmAB stain).Five cases rich in bacteria were selected for transmission electron microscopy.Results Under electron microscope,the organisms Bartonella henselae appeared polymorphic,round,elliptical,short rod or bacilliform shapes,ranged from 0.489 to 1.110 μm by 0.333 to 0.534 μm and often clustered together.Black short rod-shaped bacilli arranged in chains or clumps were demonstrated in 61.0%(47/77)of CSD by WS stain.The organisms were located outside the cells and lie mainly in the necrotic debris,especially near the nodal capsule.In 72.7%(56/77)of the cases,dot-like,granular as well as few linear positive signals were observed using BhmAB immunostain and showed similar localization.Positive results for both stains were identified in 59.7%(46/77)of the cases.When applying both stains together,Bartonella henselae was observed in 74.0%(57/77)of the earle.The difference between the results obtained by WS stain and BhmAB immunostain was of statistical significance(P＜0.05).Conclusions Bartonella henselae is the causative pathogen of cat scratch disease.WS stain,BhmAB immunostain and transmission electron microscopy are helpful in confirming the histologic diagnosis.Immunostaining using BhmAB can be a better alternative than WS stain in demonstrating the organisms.
