CAJ | 학술논문

采用酶解-超声偶联新工艺对葛根中总异黄酮提取进行优化,以提高其收率.在单因素试验的基础上,利用Box-Behnken响应面试验设计,以蒸馏水作为提取溶剂,对温度、酶浓度、时间进行三因素三水平的试验设计优化.结果表明最佳提取工艺条件为:温度34℃、酶浓度0.63 mg/mL、时间62 min.在此条件下,葛根总异黄酮提取率为7.10％,与预测值的相对误差为0.34％.酶解-超声偶联工艺是一种新型、快速、有效的总黄酮提取方法.
채용매해-초성우련신공예대갈근중총이황동제취진행우화,이제고기수솔.재단인소시험적기출상,이용Box-Behnken향응면시험설계,이증류수작위제취용제,대온도、매농도、시간진행삼인소삼수평적시험설계우화.결과표명최가제취공예조건위:온도34℃、매농도0.63 mg/mL、시간62 min.재차조건하,갈근총이황동제취솔위7.10％,여예측치적상대오차위0.34％.매해-초성우련공예시일충신형、쾌속、유효적총황동제취방법.
To increase the extraction yield of total isoflavonoids (TI) from Radix Puerariae,a novel method of cellulase coupling with ultrasound assisted extraction (UAE) was developed.The effects of extraction time,cellulase concentration,and temperature on the yield of TI were studied in single factor experiments.Subsequently,response surface methodology (RSM) was employed to optimize the extraction conditions using distilled water as extraction solvent.A regression model for the yield was established using a Box-Behnken design involving the above three factors at three levels.The experimental results showed the optimum extracton conditions as follows:extraction time of 62 min,enzyme concentration of 0.63 g/mL,temperature of 34 ℃.Under these conditions,the extractions sufficiently reached the maximal TI yield 7.10％.It suggested that the new extraction method is a reliable and potential technology which can be applied in the extraction of TI from Radix Puerariae.