CAJ | 학술논문

为了构建基于微卫星标记的中国荷斯坦种公牛亲子鉴定及个体识别技术平台，本研究选取国际动物遗传学会（ISAG）推荐的12个微卫星标记（BM1824，BM2113，SPS115，ETH10，TGLA122，ETH225，INRA023，TGLA126，TGLA227，BM1818，ETH003和TGLA53），采用荧光标记毛细管电泳方法，对571头荷斯坦种公牛进行基因型检测。统计结果显示，标记的平均多态信息含量为0．700，平均杂合度为0．735，属于高多态性标记；12个标记的累积排除概率大于0．996，累积一致性概率为4．9×10^-13，将其运用于亲子鉴定和个体识别具有很强的检测效率。此外．通过分析每个标记的检测信号峰图特征，将检测结果与商业化试剂盒进行比较以及利用ISAG参考DNA样品进行等位基因统一命名等手段，建立了标准化的微卫星等位基因分型方法，并初步建立起中国荷斯坦种公牛遗传检测信息库。
위료구건기우미위성표기적중국하사탄충공우친자감정급개체식별기술평태，본연구선취국제동물유전학회（ISAG）추천적12개미위성표기（BM1824，BM2113，SPS115，ETH10，TGLA122，ETH225，INRA023，TGLA126，TGLA227，BM1818，ETH003화TGLA53），채용형광표기모세관전영방법，대571두하사탄충공우진행기인형검측。통계결과현시，표기적평균다태신식함량위0．700，평균잡합도위0．735，속우고다태성표기；12개표기적루적배제개솔대우0．996，루적일치성개솔위4．9×10^-13，장기운용우친자감정화개체식별구유흔강적검측효솔。차외．통과분석매개표기적검측신호봉도특정，장검측결과여상업화시제합진행비교이급이용ISAG삼고DNA양품진행등위기인통일명명등수단，건립료표준화적미위성등위기인분형방법，병초보건립기중국하사탄충공우유전검측신식고。
To develop a parentage analysis and identity testing system for Chinese Holstein bulls, twelve microsatellites（BM1824, BM2113, SPSll5, ETH10, TGLA122, ETH225, INRA023, TGLA126, TGLA227, BM1818, ETH3 and TGLA53）recommend by International Society of Animal Genetics （ISAG） were used to examine 571 Holstein bulls with capillary electrophoresis. Results showed that these markers were high polymorphic, whh mean polymovphic information content of 0.700 and expeeted heterozygosity of 0.735, respectively. The cumulated exclusion probability and probability of identity was 0.997 and 4.9 ×10^-13 respectively, indicating their high power for paternity analysis and identity testing. In addition, the peak pattern of each locus was characterized so as to standardize the allelic assignment. This study facilitated future application of paternity testing and the development of a genotype database of Chinese Hostein bulls.