CAJ | 학술논문

从新疆和北京两地的十余种经济植物中的4种植物的根部分离筛选到6株可向培养基中分泌橙黄色色素物质的根内内生菌和根表附生菌，采用BOX—PCR基因组指纹图谱扩增技术对这6株细菌的遗传多样性进行了分析，结果表明这6株细菌可以划分为3个遗传群。采用系统发育和表型分析相结合对这3个遗传群的代表菌株的分类地位进行了鉴定，结果表明3个代表菌株均为绿针假单胞菌致金色假单胞菌亚种（P$eUdomonoschlororo础如subsp．aureofaciens）。采用薄层层析对3个代表菌株的色素分泌物组成进行了分析，结果显示3个代表菌株的色素分泌物组成完全相同。进一步采用电喷雾质谱和核磁共振分析确认色素主要组分为吩嗪-1-羧酸（Phenazine-1．carboxylicAcid，PCA），并对其抗真菌活性进行了分析。结论：植物根部产吩嗪羧酸抗生素的致金色假单胞菌在菌株水平上存在着显著的多样性，这种多样性与其地理分布的多样性和宿主植物的多样性之间存在着一定程度的对应关系，其中的3株内生细菌LJ1、GL1、TC1由于与宿主植物存在极为密切的关系而有可能作为生物活体农药直接应用于宿主植物病害的生物防治，显示了很好的应用潜力。
종신강화북경량지적십여충경제식물중적4충식물적근부분리사선도6주가향배양기중분비등황색색소물질적근내내생균화근표부생균，채용BOX—PCR기인조지문도보확증기술대저6주세균적유전다양성진행료분석，결과표명저6주세균가이화분위3개유전군。채용계통발육화표형분석상결합대저3개유전군적대표균주적분류지위진행료감정，결과표명3개대표균주균위록침가단포균치금색가단포균아충（P$eUdomonoschlororo출여subsp．aureofaciens）。채용박층층석대3개대표균주적색소분비물조성진행료분석，결과현시3개대표균주적색소분비물조성완전상동。진일보채용전분무질보화핵자공진분석학인색소주요조분위분진-1-최산（Phenazine-1．carboxylicAcid，PCA），병대기항진균활성진행료분석。결론：식물근부산분진최산항생소적치금색가단포균재균주수평상존재착현저적다양성，저충다양성여기지리분포적다양성화숙주식물적다양성지간존재착일정정도적대응관계，기중적3주내생세균LJ1、GL1、TC1유우여숙주식물존재겁위밀절적관계이유가능작위생물활체농약직접응용우숙주식물병해적생물방치，현시료흔호적응용잠력。
Six orange-pigment-producing endophytic and epiphytie bacterial strains were isolated from four of ten kinds of economic plant root. The genetic diversity of these 6 strains was analyzed using BOX-PCR genomic fingerprint technique which divided the 6 strains into 3 genetic groups. The taxonomic position of representative strains of the three genetic groups was determined using phylogenetic and phenotypic analysis which indicated the 3 representative strains were all Pseudomonas chlororaphis subsp, aureofaciens. Thinlayer chromatography analysis showed the pigment excreta of the 3 representative strains consisted of exactly the same composition. Eleetrospray ionization mass spectrometry and nuclear magnetic resonance analysis confirmed the major pigment component as Phena- zine-1-carboxylie Acid （PCA） , and its antifungal activity was analysed also. Conclusion： There existed notable diversity at strain level of PCA-producing Aureofaciens from plant root. The strain diversity showed corresponding relationship with the geographical environment diversity and host plant diversity, among them the 3 endophytic strains LJ1, GL1 and TC1 had the potential to be processed into living-bacteria preparation and applied to bio-proteetion of host plants due to their close relationship.