CAJ | 학술논문

建立了一种检测蜂蜜中β-呋喃果糖苷酶酶活的方法，即样液中加棉籽糖作为反应底物，在pH为5．4的缓冲液中，45aC摇床200r／min条件下反应2h，测定样液中的密二糖含量。色谱条件为：Phenomenex NH2 Luan 5U 250×4．60mm；流动相：乙腈：水=80：20；进样量：10μL；流速：1．2mL／min；柱温：40℃；示差折光检测器，温度40cc。实验结果表明：β-呋喃果糖苷酶酶活在0．3—1．7U／mL范围内线性良好，相关系数R为0．998 10。相对标准偏差为1．72％（n=5），平均回收率为94．5％。该分析方法简便、准确、灵敏，重现性好，适用于蜂蜜中β-呋喃果糖苷酶酶活的检测。
건립료일충검측봉밀중β-부남과당감매매활적방법，즉양액중가면자당작위반응저물，재pH위5．4적완충액중，45aC요상200r／min조건하반응2h，측정양액중적밀이당함량。색보조건위：Phenomenex NH2 Luan 5U 250×4．60mm；류동상：을정：수=80：20；진양량：10μL；류속：1．2mL／min；주온：40℃；시차절광검측기，온도40cc。실험결과표명：β-부남과당감매매활재0．3—1．7U／mL범위내선성량호，상관계수R위0．998 10。상대표준편차위1．72％（n=5），평균회수솔위94．5％。해분석방법간편、준학、령민，중현성호，괄용우봉밀중β-부남과당감매매활적검측。
We analyzed the concentration of melibiose in the sample solution,which reacted 2h after add raffinose as reaction substrate （reaction conditions： pH 5.4 buffer solution,45℃ shaking table 200r/min）.At last,A method was developed for the determination of beta-fructofuranosidase activity in honey. The sample was separated with NH2 column （250 ×4.60mm, 5u） at 40℃, which was then eluted with 80% acetonitrile aqueous solution at a rate of 1.2mL/min,and the signal was detected by RID.The results showed that the linear range for beta-fructofuranosidase activity were in the range of 0.3-1.7 u/mL with a correlation coeficient of 0.998 10. The relative standrd deviations for beta-fructo- furanosidase activity were 1.72% （n=5） and the average recoveries were 94.5%.This method is simple, accurate, sensi- tive and better reproducibility.