蜜蜂杂志
蜜蜂雜誌
밀봉잡지
JOURNAL OF BEE
2011年
12期
21-23
,共3页
王介平%郭军%陈杨%王海燕%李睿%王永亮
王介平%郭軍%陳楊%王海燕%李睿%王永亮
왕개평%곽군%진양%왕해연%리예%왕영량
掺假蜂蜜%液相色谱法(HPLC)%β-呋喃果糖苷酶%棉籽糖
摻假蜂蜜%液相色譜法(HPLC)%β-呋喃果糖苷酶%棉籽糖
참가봉밀%액상색보법(HPLC)%β-부남과당감매%면자당
honey adulterated%high performance liquid chromatography%beta-fructofuranosidase%raffinose
建立了一种检测蜂蜜中β-呋喃果糖苷酶酶活的方法,即样液中加棉籽糖作为反应底物,在pH为5.4的缓冲液中,45aC摇床200r/min条件下反应2h,测定样液中的密二糖含量。色谱条件为:Phenomenex NH2 Luan 5U 250×4.60mm;流动相:乙腈:水=80:20;进样量:10μL;流速:1.2mL/min;柱温:40℃;示差折光检测器,温度40cc。实验结果表明:β-呋喃果糖苷酶酶活在0.3—1.7U/mL范围内线性良好,相关系数R为0.998 10。相对标准偏差为1.72%(n=5),平均回收率为94.5%。该分析方法简便、准确、灵敏,重现性好,适用于蜂蜜中β-呋喃果糖苷酶酶活的检测。
建立瞭一種檢測蜂蜜中β-呋喃果糖苷酶酶活的方法,即樣液中加棉籽糖作為反應底物,在pH為5.4的緩遲液中,45aC搖床200r/min條件下反應2h,測定樣液中的密二糖含量。色譜條件為:Phenomenex NH2 Luan 5U 250×4.60mm;流動相:乙腈:水=80:20;進樣量:10μL;流速:1.2mL/min;柱溫:40℃;示差摺光檢測器,溫度40cc。實驗結果錶明:β-呋喃果糖苷酶酶活在0.3—1.7U/mL範圍內線性良好,相關繫數R為0.998 10。相對標準偏差為1.72%(n=5),平均迴收率為94.5%。該分析方法簡便、準確、靈敏,重現性好,適用于蜂蜜中β-呋喃果糖苷酶酶活的檢測。
건립료일충검측봉밀중β-부남과당감매매활적방법,즉양액중가면자당작위반응저물,재pH위5.4적완충액중,45aC요상200r/min조건하반응2h,측정양액중적밀이당함량。색보조건위:Phenomenex NH2 Luan 5U 250×4.60mm;류동상:을정:수=80:20;진양량:10μL;류속:1.2mL/min;주온:40℃;시차절광검측기,온도40cc。실험결과표명:β-부남과당감매매활재0.3—1.7U/mL범위내선성량호,상관계수R위0.998 10。상대표준편차위1.72%(n=5),평균회수솔위94.5%。해분석방법간편、준학、령민,중현성호,괄용우봉밀중β-부남과당감매매활적검측。
We analyzed the concentration of melibiose in the sample solution,which reacted 2h after add raffinose as reaction substrate (reaction conditions: pH 5.4 buffer solution,45℃ shaking table 200r/min).At last,A method was developed for the determination of beta-fructofuranosidase activity in honey. The sample was separated with NH2 column (250 ×4.60mm, 5u) at 40℃, which was then eluted with 80% acetonitrile aqueous solution at a rate of 1.2mL/min,and the signal was detected by RID.The results showed that the linear range for beta-fructofuranosidase activity were in the range of 0.3-1.7 u/mL with a correlation coeficient of 0.998 10. The relative standrd deviations for beta-fructo- furanosidase activity were 1.72% (n=5) and the average recoveries were 94.5%.This method is simple, accurate, sensi- tive and better reproducibility.