CAJ | 학술논문

[目的]通过模拟肝、肾毒性的体外细胞试验,研究重组人乳铁蛋白是否对小鼠原代肝肾细胞具有毒性作用,为其进一步的安全性评价提供依据。[方法]采用模拟体外消化试验所得的重组人乳铁蛋白消化产物及重组人乳铁蛋白为受试物,牛乳铁蛋白为对照,以小鼠原代肝、肾细胞为平台,用MTT法检测受试物对细胞活性的半数抑制浓度（IC50）,研究细胞毒性剂量-反应关系。[结果]在设定的试验条件下,未发现重组人乳铁蛋白对肝肾细胞存在靶毒性,结果能与已进行的亚慢性毒性试验相互印证。[结论]为对重组人乳铁蛋白的进一步安全性评价及其他转基因食品的安全性评价方法提供了参考。
[목적]통과모의간、신독성적체외세포시험,연구중조인유철단백시부대소서원대간신세포구유독성작용,위기진일보적안전성평개제공의거。[방법]채용모의체외소화시험소득적중조인유철단백소화산물급중조인유철단백위수시물,우유철단백위대조,이소서원대간、신세포위평태,용MTT법검측수시물대세포활성적반수억제농도（IC50）,연구세포독성제량-반응관계。[결과]재설정적시험조건하,미발현중조인유철단백대간신세포존재파독성,결과능여이진행적아만성독성시험상호인증。[결론]위대중조인유철단백적진일보안전성평개급기타전기인식품적안전성평개방법제공료삼고。
[Objective] The aim was to study whether recombinant Human Lactoferrin has toxic effect on Primary Hepatocyte and Nephrocyte Cell of rat to provide reference for further safety evaluation.[Method] Recombinant Human Lactoferrin and its digested products were taken as tested compound,cow Lactoferrin was used for contrast.Primary Hepatocyte and Nephrocyte Cell of rat were cultured and IC50 values were tested by MTT,and cytotoxic dose-response relationship was tested.[Result]Target toxicity was not found from recombinant Human Lactoferrin on hepatocytes and nephrocytes,in accordance with sub-chronic toxicity test.[Conclusion] This study is of reference value for further safety evaluation of recombinant Human Lactoferrin and safety of evaluation method of GM food.