CAJ | 학술논문

[目的]研究了转双价基因棉SGK321对棉蚜Aphis gossypii Glover羧酸酯酶和乙酰胆碱酯酶比活力的影响,为转基因棉的生物安全性评价提供理论依据。[方法]在SGK321及其亲本棉石远321上长期饲养棉蚜,研究2种棉花品种对棉蚜短期（第1、2、3代）和长期（第41、42、43代）的影响。[结果]在SGK321上取食1代的棉蚜种群羧酸酯酶比活力显著高于在亲本棉上取食1、2、3代的棉蚜种群,转双价基因棉SGK321上饲养1、2、3代的棉蚜乙酰胆碱酯酶比活力均显著高于在石远321上饲养相同代数的棉蚜。但长期饲养的2种棉蚜之间羧酸酯酶比活力、乙酰胆碱酯酶比活力无显著差异。[结论]长期取食转双价基因棉SGK321的棉蚜通过棉蚜解毒酶的调节作用对SGK321产生了适应性。
[목적]연구료전쌍개기인면SGK321대면아Aphis gossypii Glover최산지매화을선담감지매비활력적영향,위전기인면적생물안전성평개제공이론의거。[방법]재SGK321급기친본면석원321상장기사양면아,연구2충면화품충대면아단기（제1、2、3대）화장기（제41、42、43대）적영향。[결과]재SGK321상취식1대적면아충군최산지매비활력현저고우재친본면상취식1、2、3대적면아충군,전쌍개기인면SGK321상사양1、2、3대적면아을선담감지매비활력균현저고우재석원321상사양상동대수적면아。단장기사양적2충면아지간최산지매비활력、을선담감지매비활력무현저차이。[결론]장기취식전쌍개기인면SGK321적면아통과면아해독매적조절작용대SGK321산생료괄응성。
[Objective] The research aimed to assess the effect of transgenic Bt plus CpTI cotton variety SGK321 on carboxylesterase and acetylcholinesterase of cotton aphid Aphis gossypii and provide theoretical basis for studying the biosafety of transgenic cotton.[Method] Cotton aphids were fed with SGK321 and Shiyuan321（normal parental varieties） for over 40 generations.Enzyme activities were compared between cotton aphids feeding on SGK321 for 1,2,3,41,42 and 43 generations with those on Shiyuan321.[Result] The carboxylesterase activity of cotton aphids feeding on SGK321 for 1 generation was significantly higher than those feeding on Shiyuan321.Acetylcholinesterase activity of cotton aphids feeding on SGK321 for 1,2 and 3 generations were significantly higher than those feeding on Shiyuan321 in the same generation.But there was no significant difference of enzyme activity between cotton aphids feeding on SGK321 for a long term and those feeding on parental cotton.[Conclusion] The cotton aphid that feeding on transgenic Bt plus CpTI cotton SGK321 for a long time has adaptivity to SGK321 by regulating the detoxifying enzyme.