CAJ | 학술논문

利用基因组DNA纯化试剂盒稳定、快捷地提取单只大型溞（Daphnia magna）基因组DNA.以提取出的DNA为模板,利用线粒体DNA细胞色素氧化酶I亚基基因（COI）特异性引物,通过降落PCR技术扩增大型溞COI部分基因的序列.序列分析表明,此基因确为大型溞COI部分基因片段,从而证明本研究所提取的线粒体DNA能够为进一步开展大型溞的种群遗传学研究、物种间的亲缘关系和系统进化及种质资源管理与保护等提供物质基础.
이용기인조DNA순화시제합은정、쾌첩지제취단지대형소（Daphnia magna）기인조DNA.이제취출적DNA위모판,이용선립체DNA세포색소양화매I아기기인（COI）특이성인물,통과강락PCR기술확증대형소COI부분기인적서렬.서렬분석표명,차기인학위대형소COI부분기인편단,종이증명본연구소제취적선립체DNA능구위진일보개전대형소적충군유전학연구、물충간적친연관계화계통진화급충질자원관리여보호등제공물질기출.
We extracted DNA from single Daphnia magna using TIANamp genomic DNA purification kit,and amplified partial COI sequence using the extracted DNA as a template.The obtained sequence shares high similarity with COI sequence of GenBank,showing that it is a COI sequence of Daphnia magna specie.This study will provide data for Daphnia magna population genetics research,relationgship between species,system evolution,and the management and protection germplasm resource.