CAJ | 학술논문

本研究中,通过隐马尔科夫模型(HMM)和杨树蛋白质库搜索,共找到17个杨树铵转运体蛋白(PtAMTs).利用生物信息学方法,我们对杨树家族17条AMT蛋白序列的系统发生和AMT基因组定位进行分析,然后对其氨基酸组成成分、理化性质以及二级结构进行预测和分析,同时还分析了杨树与拟南芥、水稻、番茄、百脉根和欧洲油菜的AMT基因家族之间的联系.二级结构预测结果发现不同成员间氨基酸数目、氨基酸序列间的疏水性存在一定的差异;α-螺旋和无规则卷曲为主要二级结构组成部分.同源性比对分析表明,PtAMT基因家族主要分为2个亚家族,AMT1 (11个成员)和AMT2 (6个成员),基因结果分析表明AMT2亚家族成员不含内含子.杨树AMT蛋白的亚细胞定位分析表明PtAMT主要定位于膜结构上.电子表达图谱分析结果表明:只有XP_002309151和 XP_002334025基因有对应的EST序列,并有相应的电子表达谱,并主要在花蕾表达.
본연구중,통과은마이과부모형(HMM)화양수단백질고수색,공조도17개양수안전운체단백(PtAMTs).이용생물신식학방법,아문대양수가족17조AMT단백서렬적계통발생화AMT기인조정위진행분석,연후대기안기산조성성분、이화성질이급이급결구진행예측화분석,동시환분석료양수여의남개、수도、번가、백맥근화구주유채적AMT기인가족지간적련계.이급결구예측결과발현불동성원간안기산수목、안기산서렬간적소수성존재일정적차이;α-라선화무규칙권곡위주요이급결구조성부분.동원성비대분석표명,PtAMT기인가족주요분위2개아가족,AMT1 (11개성원)화AMT2 (6개성원),기인결과분석표명AMT2아가족성원불함내함자.양수AMT단백적아세포정위분석표명PtAMT주요정위우막결구상.전자표체도보분석결과표명:지유XP_002309151화 XP_002334025기인유대응적EST서렬,병유상응적전자표체보,병주요재화뢰표체.
Using Hidden Markov Model (HMM) scan strategy and searching the protein database, 17 ammonium transport proteins (PtAMTs) were identified from the genome of Populus trichocarpa (hereafter P. trichocarpa). Utilizing bioinformatic methods, we carried out phylogeny analyses and genome localization of the 17 AMT proteins, and analyzed their amino acid composition, physical and chemical characteristics as well as secondary structure prediction. Moreover, we assayed the evolutionary relationship of AMT gene families among P. trichocarpa and Arabidopsis thaliana (A. thaliana), Oryza sativa (O. sativa), Lycopersicon esculentum (L. esculentum), Lotus japonicus (L. japonicus), and Brassica napus (B. napus). Secondary structure assay revealed that hydrophobic amino acid sequences varied during the different PtAMTs and alpha helix and random coil were the two mainly predicted patterns. Homology alignment showed that PtAMTs were classified into 2 sub-families, 11 members in AMT1 and 6 in AMT2. Structural analyses of extrons and introns showed that all AMT2 members had no introns. Subcellular localization of protein analysis showed that: All 17 VvAMTs had delicate distribution, and were mainly located in the plasma membrane. EST analyses showed that only XP_002309151 and XP_002334025 had corresponding EST sequences and electronic expression profiles, which presumably expressed in bud.