CAJ | 학술논문

目的了解猴牙髓及其胶原对物理、化学刺激的反应。方法观察磷酸锌粘固剂及氧化锌丁香油酚粘固剂(zinc oxide eugenol，ZOE)间接盖髓后，恒河猴牙髓即刻、3 d、7 d、1个月、3个月HE和胶原特殊染色后的组织学变化。结果①磷酸锌粘固剂组术后7 d～3个月，牙髓以局限性炎症损伤为主；②ZOE组术后1～3个月胶原减少明显下降，已无炎症细胞浸润；③胶原特殊染色法可清楚地反映牙髓胶原的形态特点、分布及变化，术后3 d胶原明显减少早于炎症细胞的出现。结论磷酸锌粘固剂对牙髓产生化学刺激作用；ZOE可促进损伤牙髓的修复；胶原可作为更敏感的指标，指示牙髓的损伤和修复。
목적료해후아수급기효원대물리、화학자격적반응。방법관찰린산자점고제급양화자정향유분점고제(zinc oxide eugenol，ZOE)간접개수후，항하후아수즉각、3 d、7 d、1개월、3개월HE화효원특수염색후적조직학변화。결과①린산자점고제조술후7 d～3개월，아수이국한성염증손상위주；②ZOE조술후1～3개월효원감소명현하강，이무염증세포침윤；③효원특수염색법가청초지반영아수효원적형태특점、분포급변화，술후3 d효원명현감소조우염증세포적출현。결론린산자점고제대아수산생화학자격작용；ZOE가촉진손상아수적수복；효원가작위경민감적지표，지시아수적손상화수복。
Objective　To investigate the change of pulp and its collagen to outside stimuli. Methods　51 permanent teeth from 10 adult healthy Macaca Mulatta′s monkeys were chosen as experimental teeth. 39 of them were prepared Class V cavities. The teeth were divided into two groups, one was filled with zinc phosphate cement and another group with zinc oxide eugenol. A blank control group of 12 teeth was set at the same time. Just after the operation, 3 d, 7 d, 1 month and 3 months later, the animals were executed. Histologic changes were evaluated by means of HE and collagen staining method. Results　Local inflammation was the major representation in the pulps of the zinc phosphate cement group from 7d to 3 months after operation. The percentage of collagen decrease dropped sharply,and there was no inflammation cells infiltration in the pulps of zinc oxide eugenol group from 1 to 3 months after operation. By means of collagen staining method, the morphological characters ,distribution and the change of pulpal collagen during a certain pathologic process were clearly represented in histologic slices. The change of collagen appeared earlier than the inflammation cells infiltration. Conclusions　Zinc phosphate cement can lead to medium inflammation of the pulp. The change of collagen appears earlier than that of WBC, which can be used as a sensitive indicator.