CAJ | 학술논문

为了研究三氯化铬与大鼠胰岛细胞共培养对胰岛细胞活性与胰岛素分泌水平的影响,并对其影响机制进行探讨,以台盼蓝染色法和MTT法检测胰岛β细胞活性,以不同浓度葡萄糖刺激胰岛素释放评价三氯化铬对胰岛细胞功能的影响,同时以RT-PCR检测胰岛细胞的抗凋亡基因bcl-2的表达.结果发现,随培养液中三氯化铬浓度的增加,胰岛活性随之增加,凋亡率降低,但2.0 μmol/L的活性有所降低,凋亡率与对照组没有显著差异(P>0.05).低糖和高糖浓度环境中试验组与对照组的胰岛素分泌没有显著差异(P<0.05),RT-PCR发现试验组的bcl-2表达显著高于对照组(P<0.05).2.0 μmol/L CrCl_3明显降低胰岛细胞的凋亡率,提高其存活率,改善胰岛功能.
위료연구삼록화락여대서이도세포공배양대이도세포활성여이도소분비수평적영향,병대기영향궤제진행탐토,이태반람염색법화MTT법검측이도β세포활성,이불동농도포도당자격이도소석방평개삼록화락대이도세포공능적영향,동시이RT-PCR검측이도세포적항조망기인bcl-2적표체.결과발현,수배양액중삼록화락농도적증가,이도활성수지증가,조망솔강저,단2.0 μmol/L적활성유소강저,조망솔여대조조몰유현저차이(P>0.05).저당화고당농도배경중시험조여대조조적이도소분비몰유현저차이(P<0.05),RT-PCR발현시험조적bcl-2표체현저고우대조조(P<0.05).2.0 μmol/L CrCl_3명현강저이도세포적조망솔,제고기존활솔,개선이도공능.
To explore the mechanism and the influence of chromium chloride on the activity and insulin secretion of rat pancreatic islet cell, the activity of islet βcell was measured with MTT and trypan, and the insulin secretion was tested to evaluate the function of islet cell. The expression of antiapoptosis gene bcl-2 in islet cell was measured with RT - PCR. The results indicated that the activity of islet cell improved with the concentration of chromium chloride, but the ratio of apoptosis decreased. However, the activity of 2. 0 μmol/L chromium chloride decreased considerably, and the apoptosis ratio was not significantly different(P>0. 05). The expression of bcl-2 was significantly higher than the control group(P<0. 05). 2. 0 μmol/L chromium chloride decreased the apoptosis ratio and improved the activity of islet cell.