CAJ | 학술논문

[目的]寻找影响石楠组培苗生根的关键因子.[方法]首先比较生长素NAA、IBA及蔗糖不同浓度对石楠试管苗(2～3 cm)生根的影响,并选用IBA和NAA 2种生长素,分别设3种浓度,通过光、暗(8 d)培养对比试验,研究生长素和暗培养时间对石楠组培苗生根的影响.[结果]生根培养中适当降低蔗糖的浓度、前期暗培养8 d能显著提高石楠组培苗的生根率和生根数.生长素是石楠生根所必需的,培养基中同时添加NAA和IBA有利于根的分化,石楠组培苗在1/2 MS + IBA 0.5 mg/L + NAA 0.5 mg/L +蔗糖20 g/L培养基中生根率和生根数最高,分别为75.7%和6.9. [结论]为石楠规模化生产和转基因育种提供了理论依据.
[목적]심조영향석남조배묘생근적관건인자.[방법]수선비교생장소NAA、IBA급자당불동농도대석남시관묘(2～3 cm)생근적영향,병선용IBA화NAA 2충생장소,분별설3충농도,통과광、암(8 d)배양대비시험,연구생장소화암배양시간대석남조배묘생근적영향.[결과]생근배양중괄당강저자당적농도、전기암배양8 d능현저제고석남조배묘적생근솔화생근수.생장소시석남생근소필수적,배양기중동시첨가NAA화IBA유리우근적분화,석남조배묘재1/2 MS + IBA 0.5 mg/L + NAA 0.5 mg/L +자당20 g/L배양기중생근솔화생근수최고,분별위75.7%화6.9. [결론]위석남규모화생산화전기인육충제공료이론의거.
[Objective] The key factor affecting the rooting of Photinia serrulate was experimented.[Method] The impact of the plant growth regulator(IBA and NAA with 3 concentrations,respectively) and time of dark culture on the rooting of Photinia serrulate was analyzed based on the experiment in the different concentrations of IBA,NAA and sucrose affecting the rooting of Photinia serrulate with the height of 2-3 cm.[Results] The number and rate of its plantlet rooting could be significantly improved while the concentration of sucrose in rooting medium was appropriately reduced and the time of dark pre-culture was 8 days.The plant growth regulator is necessary for Photinia serrulate rooting and both NAA and IBA added in the medium were conducive to the root regeneration of the plant.The highest rate(75.7%) and number(6.9 root) of Photinia serrulate rooting appeared in the culture medium: 1/2 MS+IBA 0.5 mg/L+NAA 0.5 mg/L+sucrose 20 g/L.[Conclusion] The theoretical basis of large-scale production and transgenic breeding of Photinia serrulate was provided.