CAJ | 학술논문

冷诱导RNA结合蛋白(Cold inducible RNA-binding protein,CIRP)在多种冷应激细胞(包括重组中国仓鼠卵巢细胞)中被发现.迄今为止,冷应激对活体生物基凶表达的影响还未见报道.和细胞相比,生物体具有更加复杂的冷应激调节机制.本研究以冷处理的BALB/C鼠为实验动物,从其睾丸组织巾克隆出了CIRP的cDNA.结果表明,CIRP在生物体中能够被低温诱导,可能防止生物体遭受冷损伤.根据克隆的cDNA所推测的氨基酸序列与GenBank上公布的小鼠、大鼠、人类、牛蛙、美西螈、非洲爪蟾胚胎细胞和卵母细胞的CIRP氨基酸序列同源性分别为100%、99.40%、95.5%、67.4%、58.4%、76.9%和79.1%.这表明CIRP在生物进化过程中是高度保守的,可能具有多种生理功能.因此,这一研究将为探索人类和动物冷应激分子机制创立系统试验模型和奠定新的实践基础.图5参14
랭유도RNA결합단백(Cold inducible RNA-binding protein,CIRP)재다충랭응격세포(포괄중조중국창서란소세포)중피발현.흘금위지,랭응격대활체생물기흉표체적영향환미견보도.화세포상비,생물체구유경가복잡적랭응격조절궤제.본연구이랭처리적BALB/C서위실험동물,종기고환조직건극륭출료CIRP적cDNA.결과표명,CIRP재생물체중능구피저온유도,가능방지생물체조수랭손상.근거극륭적cDNA소추측적안기산서렬여GenBank상공포적소서、대서、인류、우와、미서원、비주조섬배태세포화란모세포적CIRP안기산서렬동원성분별위100%、99.40%、95.5%、67.4%、58.4%、76.9%화79.1%.저표명CIRP재생물진화과정중시고도보수적,가능구유다충생리공능.인차,저일연구장위탐색인류화동물랭응격분자궤제창립계통시험모형화전정신적실천기출.도5삼14
The cold-inducible RNA-binding protein (CIRP) was found in various cells including recombinant Chinese hamster ovary (rCHO) cells under cold stress. However, the effect of cold stress on the gene expression of the intravital animals has not been reported till now. Compared with their cells, there were much more complicated regulatory mechanisms for cold stress response in the organisms. The BALB/C mice with cold treatment were used as experimental animals for this study. The cDNA of CIRP was firstly cloned from the testis tissues of the BALB/C mice treated by cold stress. The results indicated that CIRP in the organisms could be induced at low temperature and might protect the organisms from the cold damage. The amino acid sequences deduced via cDNA clone were 100%, 99.4%, 95.5%, 67.4%, 58.4%,76.9%, and 79.1% identical to those of the CIRP in mice, rats, human, bullfrog and axolotl cells, and Xenopus embryos and oocytes, respectively. These results show that the CIRP is highly conserved in the evolution process and may be involved in various physiological functions. Therefore, this study will establish a systematic model for experiments and provide a new foundation for exploring the molecular mechanisms of human and animals under cold stress. Fig 5, Ref 14