上海大学学报(英文版)
上海大學學報(英文版)
상해대학학보(영문판)
JOURNAL OF SHANGHAI UNIVERSITY (ENGLISH EDITION)
2007年
6期
619-624
,共6页
王红梅%储黄伟%刘海生%李晓星%杨功达%张大兵%雍克岚
王紅梅%儲黃偉%劉海生%李曉星%楊功達%張大兵%雍剋嵐
왕홍매%저황위%류해생%리효성%양공체%장대병%옹극람
rice (Oryza sativa L.)%glume%OsEG1%spikelet meristem%primary mapping.
A rice mutant with Japonica 9522 cultivar background Oryza sativa extraordinary glume 1 (Oseg 1) was identified from the M2 mutant pool mutagenized by 60Co γ-ray. Compared with wild type plants, Oseg 1 developed longer empty glumes and rudimentary glumes. In some Oseg 1 mutants, the number of stamens of flowers was reduced and leaf-like lodicules occurred, and excessive lemma/palea-like organ could be observed in some mutant spikelets. This indicated that OsEG1 could regulate the development of rudimentary glumes, empty glumes, lemma/palea, lodicules, and stamens. Genetic analysis indicated that Oseg 1 came from a single recessive genetic locus. To clone OsEG1 gene, F2 population was constructed by a cross between Oseg 1 (Japonica) and Guangluai4 (Indica). Using map-based cloning approach, OsEG1 was mapped on chromosome 4, between INDEL marker OS407 and WHM0466 with genetic distance of 2.0 cm and 1.0 cm, respectively. These results are useful for further cloning and functional analysis of the OsEG1 gene.