CAJ | 학술논문

目的探讨TOLL样受体4(TLR4)在脑缺血再灌注中的保护作用.方法 C3H/HeJ小鼠16只采用线栓法制作TLR4先天缺损大脑中动脉缺血再灌注模型(C3H/HeJ组),以C3H/HeN小鼠16只作对照(C3H/HeN组),观察缺血6 h及再灌注24 h时,两组小鼠神经功能缺损评分、脑含水量、脑梗死体积及血清TNF-α水平.光镜及电镜观察两者缺血再灌注脑组织损伤情况.结果与C3H/HeN组比较,再灌注24 h时,C3H/HeJ组小鼠的神经功能缺损评分明显减小(P＜0.05);脑含水量明显降低,脑梗死体积显著缩小(P＜0.01);血清中TNF-α浓度明显降低(P＜0.05);脑缺血皮质区的神经细胞超微结构改变轻于C3H/HeN组小鼠.结论 C3H/HeJ小鼠脑缺血再灌注损伤明显减轻,TLR4介导了脑缺血再灌注损伤.其机制可能与炎性细胞因子减少有关.
목적 탐토TOLL양수체4(TLR4)재뇌결혈재관주중적보호작용.방법 C3H/HeJ소서16지채용선전법제작TLR4선천결손대뇌중동맥결혈재관주모형(C3H/HeJ조),이C3H/HeN소서16지작대조(C3H/HeN조),관찰결혈6 h급재관주24 h시,량조소서신경공능결손평분、뇌함수량、뇌경사체적급혈청TNF-α수평.광경급전경관찰량자결혈재관주뇌조직손상정황.결과 여C3H/HeN조비교,재관주24 h시,C3H/HeJ조소서적신경공능결손평분명현감소(P＜0.05);뇌함수량명현강저,뇌경사체적현저축소(P＜0.01);혈청중TNF-α농도명현강저(P＜0.05);뇌결혈피질구적신경세포초미결구개변경우C3H/HeN조소서.결론 C3H/HeJ소서뇌결혈재관주손상명현감경,TLR4개도료뇌결혈재관주손상.기궤제가능여염성세포인자감소유관.