CAJ | 학술논문

　　目的：观察注射用重组人肿瘤坏死因子受体-抗体融合蛋白（益赛普，Etanercept）对异丙肾上腺素引起的小鼠腮腺腺肿大的影响，进一步探究其腮腺肿大的机制。方法：将21只雄性昆明小鼠随机分为三组：正常对照组（7只）、异丙肾上腺素（IPR）注射组（7只）和益赛普治疗组（7只）。IPR组和益赛普组按30 mg/kg体重每天腹腔注射IPR，连续注射6 d。益赛普治疗组同时皮下注射益赛普15 mg/kg体重，共计2次。对照组小鼠注射同等体积的生理盐水。第7天处死动物，取腮腺组织制片，HE染色观察组织学变化及进行免疫组化观察组织中TNF-α和IL-1β变化；使用酶联免疫吸附分析法检测腮腺组织中TNF-α和IL-1β水平。结果：病理组织学上IPR组与益赛普治疗组腺细胞的增生和肿大比较无明显差异；IPR组小鼠腮腺组织中TNF-α和IL-1β水平与对照组相比明显升高；益赛普治疗组TNF-α和IL-1β水平与对照组相比，也明显增高，与IPR组比较差异有统计学意义（P<0.05）。在免疫组化方面，与对照组相比，IPR组与益赛普治疗组TNF-α和IL-1β表达都有所增加（P<0.05），但益赛普治疗组TNF-α和IL-1β的表达的强度有所减弱。结论：在连续注射IPR引起小鼠腮腺肿大的机制中，尽管腮腺组织TNF-α和IL-1β含量增多，益赛普部分阻断腮腺组织中TNF-α和IL-1β的含量，但不能明确两种细胞因子的增多与腮腺肿大有必然的关系，需要进一步探讨。
　　목적：관찰주사용중조인종류배사인자수체-항체융합단백（익새보，Etanercept）대이병신상선소인기적소서시선선종대적영향，진일보탐구기시선종대적궤제。방법：장21지웅성곤명소서수궤분위삼조：정상대조조（7지）、이병신상선소（IPR）주사조（7지）화익새보치료조（7지）。IPR조화익새보조안30 mg/kg체중매천복강주사IPR，련속주사6 d。익새보치료조동시피하주사익새보15 mg/kg체중，공계2차。대조조소서주사동등체적적생리염수。제7천처사동물，취시선조직제편，HE염색관찰조직학변화급진행면역조화관찰조직중TNF-α화IL-1β변화；사용매련면역흡부분석법검측시선조직중TNF-α화IL-1β수평。결과：병리조직학상IPR조여익새보치료조선세포적증생화종대비교무명현차이；IPR조소서시선조직중TNF-α화IL-1β수평여대조조상비명현승고；익새보치료조TNF-α화IL-1β수평여대조조상비，야명현증고，여IPR조비교차이유통계학의의（P<0.05）。재면역조화방면，여대조조상비，IPR조여익새보치료조TNF-α화IL-1β표체도유소증가（P<0.05），단익새보치료조TNF-α화IL-1β적표체적강도유소감약。결론：재련속주사IPR인기소서시선종대적궤제중，진관시선조직TNF-α화IL-1β함량증다，익새보부분조단시선조직중TNF-α화IL-1β적함량，단불능명학량충세포인자적증다여시선종대유필연적관계，수요진일보탐토。
Objective:To study the effects of etanercept on enlargement of parotid glands induced by IPR in mice,and to explore the mechanism of enlargement of parotid glands.Method:Twenty-one male kunming mice were divided into 3 groups:control group,IPR group and treatment group administered with rhTNFR:Fc.Mice in the IPR group and treatment group were injected with isoproterenol intraperitoneally at a dose of 30 mg/kg once for consecutive six days.At the same time,treatment group mice were administered subcutaneously etanercept twice at a dose of 15 mg/kg while mice in the control group were injected with the same volume of saline. The next day after all the treatments,mice were killed by exsanguination. Samples of parotid glands were observed by using HE staining. Expressions of TNF-αand IL-1βin parotid glands were determined by immunohistochemistry and by ELISA. Result:Expressions of TNF-αand IL-1βin parotid glands were increased significantly after treatments in the IPR group and treatment group compared with the control group,and there was a significant difference between IPR group and treatment group(P<0.05).Immunohistochemistry showed that the expressions of TNF-αand IL-1βin treatment group were weaker than those in IPR group.No marked changes were observed in histopathology between IPR group and treatment group.Conclusion:Though we observed different expressions of TNF-αand IL-1βin treatment group and IPR group,we could not confirm that if there is an inevitable relation between the enlargement of its mechanism and the increase of TNF-αand IL-1β,and need further study.