CAJ | 학술논문

目的:研究围手术期异体输血对胃癌患者Th细胞极化的影响.方法:胃癌根治手术患者,接受围手术期异体输血.用流式细胞术检测TH细胞极化情况.结果:A组术后2天与术前比较TH1细胞减少,Th2细胞增加,至术后5天恢复到术前水平.B组、C组输血后Th1细胞和Th1/Th2比值恢复到术前水平,但C组仍较低(P＜0.05).与A组比较C组Th2细胞增加,Th1细胞和Th1/Th2比值下降比B组更显著(P＜0.05).结论:围手术期异体输输血优于输注全血.Th细胞向Th2细胞方向极化可能是异体输血后引起免疫抑制的原因.
목적:연구위수술기이체수혈대위암환자Th세포겁화적영향.방법:위암근치수술환자,접수위수술기이체수혈.용류식세포술검측TH세포겁화정황.결과:A조술후2천여술전비교TH1세포감소,Th2세포증가,지술후5천회복도술전수평.B조、C조수혈후Th1세포화Th1/Th2비치회복도술전수평,단C조잉교저(P＜0.05).여A조비교C조Th2세포증가,Th1세포화Th1/Th2비치하강비B조경현저(P＜0.05).결론:위수술기이체수수혈우우수주전혈.Th세포향Th2세포방향겁화가능시이체수혈후인기면역억제적원인.
Objective: To study the effects of allogeneic blood transfusion on polarized Th cell in gastric carcinoma patients during perioperative time. Methods: Thirty patients undergoing gastric carcinectomy were randomly divided into three groups with 10 patients in each group: group A received no allogeneic blood; group B received leukodepleted blood; group C received allogeneic whole blood during perioperative time. The polarized rates of Th cell were measured by flow cytometry. Results: The counts of Th1 in group A decreased significantly on the 2nd postoperation, but returned to the baseline levels on the 5th postoperative day. The Th1 counts and Th1/Th2 ratio decreased significantly in group B and group C after blood transfusion (P ＜ 0.05 ). The Th2 counts increased significantly in group B and C postoperative time. On the 10th postoperative day the Th1 、Th2 cell counts and Th1/Th2 ratio returned to preoperative level in group B, but remained low in group C. As compared with that in group A, the Th1 cell counts, Th1/Th2 ratio reduced and Th2 cell counts increased markedly in group C than in group B (P ＜ 0.05 ). Conclusion: Perioperative blood transfusion contributes to the immunosuppression, which is more serious after whole blood transfusion than leukodepleted blood. In perioperative period component blood transfusion is superior to whole blood transfusion. The Th cells polarized to Th2 cells may be one of the causes of immunosuppression in patients received allogeneic blood transfusion.