中国科学C辑(英文版)
中國科學C輯(英文版)
중국과학C집(영문판)
SCIENCE IN CHINA (Series C)
2003年
3期
235-242
,共8页
李园莉%孙杰%李春红%朱勇清%夏桂先
李園莉%孫傑%李春紅%硃勇清%夏桂先
리완리%손걸%리춘홍%주용청%하계선
cotton%microtubules%β-tubulin%fiber development
A cDNA library was constructed using poly (A)+ RNA isolated from -1-15 DPA fibers of upland cotton (Gossypium hirsutum). The cDNA encoding a β-tubulin isoform (designated as GhTub1) was identified through EST search. Northern blot analysis using 3′-UTR of the cDNA as a gene-specific probe was performed to investigate the expression levels of GhTub1 in various organs and in the developing fibers. The results showed that GhTub1 gene was specifically expressed in cotton fiber cells. During fiber development, GhTub1 transcripts accumulated highlyat the stage of cell rapid elongation with the highest expression appearing at the time when fiber expansion reaches the peak rate. To probe the in vivo function of GhTub1, its cDNA was cloned in the yeast expression vector pREP1 and transformed into the fission yeast Schizosaccharomyces pombe. Overexpression of GhTub1 in yeast cells caused severe changes in the cell morphology. These results suggest that GhTub1 may play a role in the polar elongation of cotton fibers. To our knowledge, this is the first report on the fiber-specific transcript accumulation of a cotton β-tubulin gene.