CAJ | 학술논문

目的：对不同浓度尼可地尔心肌麻痹液超极化心脏停搏心肌保护的剂量-效应关系进行研究，选择最适心肌保护剂量。方法：实验分6组，即对照组(去极化心脏停搏组)、5种不同浓度(25、50、100、125和150 μmol/L)尼可地尔超极化心脏停搏组。4℃心肌麻痹液(40 ml/kg)诱停离体鼠心，每隔30 min重复灌注心肌麻痹液(20 ml/kg)，(15±1)℃心脏停搏120 min后再灌注，离体心工作30 min。记录心脏机械停搏时间、再灌注后心脏复跳情况、心功能恢复(左室发展压、主动脉流量及左室压力微分)、冠状循环灌注平均流量、心肌丙二醛(MDA)含量、心肌超微结构的改变。结果：尼可地尔心肌麻痹液心肌保护呈类似抛物线形的剂量-效应关系。尼可地尔浓度为100 μmol/L时，LVDP的恢复率最佳，心肌保护效果最好。结论：尼可地尔超极化心脏停搏心肌保护的最适心肌保护剂量为100 μmol/L。
목적：대불동농도니가지이심기마비액초겁화심장정박심기보호적제량-효응관계진행연구，선택최괄심기보호제량。방법：실험분6조，즉대조조(거겁화심장정박조)、5충불동농도(25、50、100、125화150 μmol/L)니가지이초겁화심장정박조。4℃심기마비액(40 ml/kg)유정리체서심，매격30 min중복관주심기마비액(20 ml/kg)，(15±1)℃심장정박120 min후재관주，리체심공작30 min。기록심장궤계정박시간、재관주후심장복도정황、심공능회복(좌실발전압、주동맥류량급좌실압력미분)、관상순배관주평균류량、심기병이철(MDA)함량、심기초미결구적개변。결과：니가지이심기마비액심기보호정유사포물선형적제량-효응관계。니가지이농도위100 μmol/L시，LVDP적회복솔최가，심기보호효과최호。결론：니가지이초겁화심장정박심기보호적최괄심기보호제량위100 μmol/L。
Objectives:Dose-response effect of nicorandil cardioplegia at various concentrations was studied to optimize its myocardial protective effect.Methods:Forty-eight isolated working rat hearts were divided into 6 groups randomly.They were group A:control (depolarized cardiac arrest with St.Thomas solution No.2),group B,C,E,F and G:hyperpolarized cardiac arrest (nicorandil concentration were 25,50,100,125 and 150μmol/L respectively).The hearts underwent a 120-minute hypothermic arrest (15±1)℃ with cardioplegia (40 ml/kg) and reinfused with cardioplegia (40 ml/kg) at interval of 30 minutes.Mechanical arrest time,cardiac functional recoveries,myocardial content of malondialdehyde (MDA) and ultrastructure were measured.Results:The protective effect of nicorandil cardioplegia was dose-related.Conclusions:The optimal concentration of nicorandil in cardiplegia may be 100μmol/L for myocardial protection.