CAJ | 학술논문

目的观察糖皮质激素(Dex)、孕激素(P)、雌激素(E)、雄激素(T)等甾体激素(10-10～10-6mol／L)对人卵巢癌细胞系3AO增殖及分化的影响。方法以不同浓度Dex、E、P、T处理培养的3AO细胞，采用活细胞计数观察细胞增殖情况，用氨基氨替比林法测定碱性磷酸酶(ALP)活性，用酶联免疫法测定3AO细胞标志抗原CA125水平的变化；采用完整细胞饱和测定法测定3AO细胞中糖皮质激素受体(GR)的表达。结果 Dex对3AO细胞的增殖有明显的抑制作用，糖皮质激素受体拮抗剂Ru486可以阻断糖皮质激素对3AO的增殖抑制作用；E、P、T等甾体激素对3AO细胞的增殖无明显作用(P>0.05)。Dex作用后ALP活性较对照明显增加，CA125水平明显降低(P<0.001)；而E、P、T作用后ALP活性无明显变化(P>0.05)，CA125较对照组明显下降(P<0.001)。3AO细胞含有可饱和性高亲和力GR，最大结合容量为92.26±23.30 fmol／10-6cells，平衡解离常数为1.37±0.19 nmol／L，特异结合实验表明，GR只与糖皮质激素特异结合。结论糖皮质激素对3AO细胞系有增殖抑制和诱导分化的作用，其调节作用通过GR来介导；E、P、T等甾体激素对3AO细胞无增殖抑制作用。
목적관찰당피질격소(Dex)、잉격소(P)、자격소(E)、웅격소(T)등치체격소(10-10～10-6mol／L)대인란소암세포계3AO증식급분화적영향。방법이불동농도Dex、E、P、T처리배양적3AO세포，채용활세포계수관찰세포증식정황，용안기안체비림법측정감성린산매(ALP)활성，용매련면역법측정3AO세포표지항원CA125수평적변화；채용완정세포포화측정법측정3AO세포중당피질격소수체(GR)적표체。결과 Dex대3AO세포적증식유명현적억제작용，당피질격소수체길항제Ru486가이조단당피질격소대3AO적증식억제작용；E、P、T등치체격소대3AO세포적증식무명현작용(P>0.05)。Dex작용후ALP활성교대조명현증가，CA125수평명현강저(P<0.001)；이E、P、T작용후ALP활성무명현변화(P>0.05)，CA125교대조조명현하강(P<0.001)。3AO세포함유가포화성고친화력GR，최대결합용량위92.26±23.30 fmol／10-6cells，평형해리상수위1.37±0.19 nmol／L，특이결합실험표명，GR지여당피질격소특이결합。결론당피질격소대3AO세포계유증식억제화유도분화적작용，기조절작용통과GR래개도；E、P、T등치체격소대3AO세포무증식억제작용。
Objective To observe the effects of glucocorticoids, estrogen, progesterone, androgen, testosteroneon the proliferation and differentiation of 3AO cells. Methods 3AO cell proliferation was evaluated by viable cellcount, alkaline phosphatase (ALP) activity was determined as described and expression was measured by ElISA. GRexpression was studied with the standard radioligand binding assay. Results Dex could inhibit the proliferation of 3AOcells, but the cellular effects of glucocorticoids on 3AO cells could be reversed by RU486 ,a potent glucocorticoid antagonist. Sex steroid hormones had little effects on the proliferation of 3AO cells. ALP activity increased significantly aftertreatment with Dex and had no effects with sex steroid hormones. Furthermore, Dex and sex steroid hormones couldsuppress the expression of tumor maker CA125 in 3AO cells. Binding studies showed that there existed a saturable,high-affinity GR in 3AO cells, with a maximum binding capacity of 92.26 + 23.30 fmol/106 cells, and a constant dissociation value of 1.37 ± 0.19 nmol/L. Conclusion Dex can inhibit the proliferation and induce the differentiation of3AO cells, and the cellular effects of glucocorticoids on 3AO cells is mediated by GR; but sex steroid hormones has noeffects on the proliferation of 3AO cells. (Shanghai Med J, 2001,24:99-101)