CAJ | 학술논문

运用31P核磁共振波谱(NMRS)研究血清中磷脂的代谢变化，并探讨该法对白血病诊断的可行性.方法采用MSL-300MHz型超导核磁共振仪测量白血病人及健康人血清标本，通过分析比较血清中磷脂酰胆碱(PC)信号及磷脂酰乙醇胺和(神经)鞘磷脂(PE+SM)信号相对积分面积，了解各自血清中的磷脂含量水平.结果，白血病人血清中的磷脂信号强度相对正常人明显偏低，反映了对应的PC及(PE+SM)含量相对正常人偏低，尤其对初发未治疗的病人，差别更加明显.结论，本法可作为研究血清中磷脂的变化、代谢情况及白血病人临床诊断的新方法.
운용31P핵자공진파보(NMRS)연구혈청중린지적대사변화，병탐토해법대백혈병진단적가행성.방법채용MSL-300MHz형초도핵자공진의측량백혈병인급건강인혈청표본，통과분석비교혈청중린지선담감(PC)신호급린지선을순알화(신경)초린지(PE+SM)신호상대적분면적，료해각자혈청중적린지함량수평.결과，백혈병인혈청중적린지신호강도상대정상인명현편저，반영료대응적PC급(PE+SM)함량상대정상인편저，우기대초발미치료적병인，차별경가명현.결론，본법가작위연구혈청중린지적변화、대사정황급백혈병인림상진단적신방법.
To investigate the feasibility of serum 31P NMRS in the study phospholipid metabolites and diagnosis of leukaemia. Method: Serum sample of leukaemia patients and healthy controls were collected and measured by 31P NMRS with MSL-300MHz spectrometer. The phospholipid contents were known by contraxt relatively integral area of PC signal and (PE+SM) signal. Results: In spectra of serum of leukaemia patients, the phospholipid signal intensities were significantly decreases. It reflected lower phosphatidylcholine and phosphatidylcholine and sphingomyelin content in serum of leukemia patients, especially for original untreatment patient. Conclusions: These results suggest that serum 31P NMRS may likely become a promising new method for the study of phospholipid changes or metabolites and identifying the presence of leukaemia.