CAJ | 학술논문

目的探讨基因工程人血管内皮抑制素 (endostatin)抑制黑素瘤在小鼠体内生长和转移的作用及其作用机制。方法接种黑素瘤细胞悬液 (2× 106/鼠 )于小鼠皮下，成瘤后给予血管内皮抑制素 (8 mg/kg· d)，每天 1次，共 21次。给药期间观察荷瘤小鼠的饮食情况、体重变化，并测量肿瘤的大小。于第 26天处死，称皮下肿瘤的重量，并取脑、肺、肝、脾和肾切片做病理学检查。结果血管内皮抑制素治疗组的曲线下面积明显小于肿瘤对照组 (P＜ 0.01)。病理切片检查显示，治疗组肿瘤有大面积的坏死，瘤周毛细血管消失。结论血管内皮抑制素对小鼠黑素瘤的生长、转移，以及新生毛细血管的形成具有明显地抑制作用。
목적탐토기인공정인혈관내피억제소 (endostatin)억제흑소류재소서체내생장화전이적작용급기작용궤제。방법접충흑소류세포현액 (2× 106/서 )우소서피하，성류후급여혈관내피억제소 (8 mg/kg· d)，매천 1차，공 21차。급약기간관찰하류소서적음식정황、체중변화，병측량종류적대소。우제 26천처사，칭피하종류적중량，병취뇌、폐、간、비화신절편주병이학검사。결과혈관내피억제소치료조적곡선하면적명현소우종류대조조 (P＜ 0.01)。병리절편검사현시，치료조종류유대면적적배사，류주모세혈관소실。결론혈관내피억제소대소서흑소류적생장、전이，이급신생모세혈관적형성구유명현지억제작용。
Aim To explore inhibitory effects and mechanism of engineering endostatin on growth and metastasis of melanoma cells in mice. Methods Melanoma cells(2× 106/mouse)were inoculated sabcutaneously to C57BL/6 mice. After tumorigenesis,endostatin(8mg/kg.d)was administrated to tumor-bearing mice,once a day ,twenty-one in all.Dietetic state and weight change of the tumor-bearing mice were observed and tumorous sige was measured during administration of endostatin. On 26thday,the tumor-bearing mice were sacrificed,subcutaneously tumorous weight was weighed and brain,lung ,liver,spleen and kidney were excised and sections were made to supply the pathological examination. Results Area under curve in the endostatin-treated group was obviously less than that in tamor control group(P∨ 0.01). Pathological study revealed that lavge areal necrosis arose in tumor and newborn cappillaries around the tumor disapeared. Conclusion Endostatin possosses strongly inhibitory effects on growth and metastasis of mouse melanoma and formation of newborn capillaries around tumor.