CAJ | 학술논문

目的探讨髓系细胞表面BST-1(骨髓基质细胞抗原1)/CD157分子能否作为一受体介导信号传导和调节细胞表面FcγR的表达。方法制备兔抗人BST-1多克隆抗体及其F(ab')2片段，以其刺激U937细胞后，用免疫沉淀及免疫印迹法研究胞内蛋白酪氨酸磷酸化水平的变化；用FACS法检测细胞表面FcγR的表达。结果抗人BST-1抗体刺激U937细胞可介导胞内多种蛋白的酪氨酸磷酸化水平增高，包括Mr为120×103的c-cbl原癌蛋白；并可下调细胞表面CD64的表达。抗BST-1抗体的这些作用呈抗体Fc段依赖性。结论髓系细胞表面BST-1分子可作为一受体介导细胞信号传导；抗BST-1抗体刺激U937细胞可下调其表面CD64的表达
목적탐토수계세포표면BST-1(골수기질세포항원1)/CD157분자능부작위일수체개도신호전도화조절세포표면FcγR적표체。방법제비토항인BST-1다극륭항체급기F(ab')2편단，이기자격U937세포후，용면역침정급면역인적법연구포내단백락안산린산화수평적변화；용FACS법검측세포표면FcγR적표체。결과항인BST-1항체자격U937세포가개도포내다충단백적락안산린산화수평증고，포괄Mr위120×103적c-cbl원암단백；병가하조세포표면CD64적표체。항BST-1항체적저사작용정항체Fc단의뢰성。결론수계세포표면BST-1분자가작위일수체개도세포신호전도；항BST-1항체자격U937세포가하조기표면CD64적표체
Purpose　To examine whether BST-1/CD157 expressed on myeloid cells can function as a receptor molecule to mediate signal into cells and regulate the expression of FcγR on cells.　Methods　The tyrosine phosphorylation of U937 cells stimulated by immunoprecipitation and immunoblotting method;FcγR expression level on U937 cells stimulated by anti-BST-antibody was analyzed by FACS.　Results　Stimulation of U937 cells by anti-BST-1 polyclonal antibody resulted in protein tyrosine phosphorylation of U937 cells,including Mr 120×103 c-cbl onco-protein,and the down-regulation of CD64 expression on U937 cells.The activities of anti-BST-1 antibody depended on its Fc portion.　Conclusions　BST-1 molecules expressed on myeloid cells can function as a receptor molecule to mediate signal into cells.Stimulation of U937 cells by anti-BST-1 antibody down-regulate the expression of CD64.