CAJ | 학술논문

用抗氧剂和螯合剂组合的方法，成功地从太白红杉的叶组织中提取和纯化了总DNA，并对其产率、质量和纯度作了鉴定。用此方法能有效地去除酚类化合物、多糖、萜类化合物和未知化合物的干扰，为太白红杉的分子生物学研究提供了一套迅速、经济和可靠的技术方案。
용항양제화오합제조합적방법，성공지종태백홍삼적협조직중제취화순화료총DNA，병대기산솔、질량화순도작료감정。용차방법능유효지거제분류화합물、다당、첩류화합물화미지화합물적간우，위태백홍삼적분자생물학연구제공료일투신속、경제화가고적기술방안。
An extraction medium with the combination of antioxidants andchelants, which can remove phenolic compound, polysaccharide and terpenoid, was successfully used to obtain total DNA from the leaf tissues of Larix chinensis beissn. The DNA yields, quality and purity were characterized. These isolated DNA could be used directly for PCR reaction, RFLP and RAPD analysis. A rapid, inexpensive and reliable procedure has been developed for further study of genetic diversity of this species.