CAJ | 학술논문

为探讨蛋白激酶在青石棉诱导的肺成纤维细胞增殖中的作用，本研究采用体外细胞培养技术，用兔肺泡巨噬细胞（AM）和人胚肺成纤维细胞（HEPF）组成体外模型，用MTT法测定HEPF的增殖活力，检测了蛋白激酶（PKA、PKC和TPK）的抑制剂和激活剂对青石棉诱导的HEPF增殖的影响，以二氧化钛为阴性对照，标准石英为阳性对照。结果发现，PKA、PKC和TPK的抑制剂和激活剂均能使青石棉诱导的HEPF增殖受到抑制和激活，并随剂量的变化而变化，呈显著的剂量-效应关系（P<0.01），且青石棉组被抑制和激活的程度强于各对照组。从3种蛋白激酶的作用强度分析，发现TPK信号通路在青石棉诱导的HEPF增殖中的作用最强，其次是PKC信号通路，PKA信号通路的作用最弱。提示PKA、PKC和TPK信号通路均参与了青石棉处理AM上清液致HEPF增殖过程，TPK信号通路可能起着主导作用，这为寻找石棉致纤维化因子的研究方向提供了参考。
위탐토단백격매재청석면유도적폐성섬유세포증식중적작용，본연구채용체외세포배양기술，용토폐포거서세포（AM）화인배폐성섬유세포（HEPF）조성체외모형，용MTT법측정HEPF적증식활력，검측료단백격매（PKA、PKC화TPK）적억제제화격활제대청석면유도적HEPF증식적영향，이이양화태위음성대조，표준석영위양성대조。결과발현，PKA、PKC화TPK적억제제화격활제균능사청석면유도적HEPF증식수도억제화격활，병수제량적변화이변화，정현저적제량-효응관계（P<0.01），차청석면조피억제화격활적정도강우각대조조。종3충단백격매적작용강도분석，발현TPK신호통로재청석면유도적HEPF증식중적작용최강，기차시PKC신호통로，PKA신호통로적작용최약。제시PKA、PKC화TPK신호통로균삼여료청석면처리AM상청액치HEPF증식과정，TPK신호통로가능기착주도작용，저위심조석면치섬유화인자적연구방향제공료삼고。
In order to study the role of protein kinase in the proliferationof lung fibroblasts induced by crocidolite. An in vitro model was established by rabbit alveolar macrophage (AM) and human embryonic pulmonary fibroblasts (HEPF). Using MTT color response method to measure HEPF proliferation, the influence of the inhibitor or activator of protein kinase (PKA, PKC and TPK) on the proliferation of crocidolite-induced HEPF were investigated. TiO2 was taken as negative control and SiO2 positive control. The results showed that the inhibitors of PKA, PKC and TPK could all inhibit the proliferation of HEPF induced by crocidolite, their activators could also promote the proliferation of HEPF. There all existed significant dose-effect relationships (P<0.01), and the intensity in crocidolite group was inhibited or activated more than that in the controls. Through acting intensity analysis, the intensity was found as follows: TPK>PKC>PKA. It was suggested that TPK, PKC and PKA signal pathways were all involved in the process of the proliferation of crocidolite-induced HEPF, but TPK maybe played a key role in this process. This study provide leads for further research on identifying the bioactive factors of proliferation of crocidolite-induce HEPF.