CAJ | 학술논문

已有研究表明,外周神经损伤后Aβ神经元的初级传入末梢在脊髓的病理性生芽与损伤后痛觉超敏的形成有关.为了证实外周神经损伤后形成的这种生芽是否具有可逆性,本研究以大鼠坐骨神经压迫造成神经源性痛模型,采用跨神经节的霍乱毒素B亚单位结合辣根过氧化物酶(CB-HRP)作为示踪剂,四甲基联苯胺(TMB)反应呈色追踪Aβ初级传入末梢.结果证实:对坐骨神经的压迫也能够导致痛觉过敏和Aβ纤维末梢生芽侵入脊髓后角Ⅱ层,并且这种生芽不伴随对神经压迫的解除而逆转.中枢神经系统的这种结构重组及其不可逆性可能是神经源性痛的产生和维持的重要原因.
이유연구표명,외주신경손상후Aβ신경원적초급전입말소재척수적병이성생아여손상후통각초민적형성유관.위료증실외주신경손상후형성적저충생아시부구유가역성,본연구이대서좌골신경압박조성신경원성통모형,채용과신경절적곽란독소B아단위결합랄근과양화물매(CB-HRP)작위시종제,사갑기련분알(TMB)반응정색추종Aβ초급전입말소.결과증실:대좌골신경적압박야능구도치통각과민화Aβ섬유말소생아침입척수후각Ⅱ층,병차저충생아불반수대신경압박적해제이역전.중추신경계통적저충결구중조급기불가역성가능시신경원성통적산생화유지적중요원인.
It is proposed that following peripheral nerve injury abnormal sprouting of Aβ fiber primary afferent neurons in the spinal cord contributes to the allodynia that often occurs with such injury. The present investigation is to determine whether this sprouting is reversal after compression of peripheral nerve was relieved. In a rat model of neuropathic pain made by rat sciatic nerve compression,chorela toxin B subunit conjugated horseradish peroxidase (CB-HRP) was used to trace the termination of Afiber primary afferents and sections were reacted for using tetramethylbenzidine (TMB) as the chromagen. We demonstrated that the compression to the sciatic nerve also results in hyperalgesia and novel transganglionic CB-HRP staining in laminae Ⅱ, and this sprouting can not be reversed by decompression. This structural reorganization in central nervous system and its irreversible character may contribute to the development and maintenance of neuropathic pain.