CAJ | 학술논문

为寻找从鱼油中分离高纯度二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的更有效方法，分别以3,5-二硝基苯甲酰氯和2-氯乙醇修饰的聚甲基丙烯酸甲酯(PMDVB)树脂微球，用作高效液相色谱(HPLC)的固定相。均比修饰前的PMDVB树脂微球分离效果好，比Waters反相C18键合硅胶柱分离因子大，柱容量高。以3,5-二硝基苯甲酰氯修饰的PMDVB树脂效果最理想，以2-氯乙醇修饰的PMDVB树脂微球，造价低，工业上具有推广价值。作者对分离机理的研究表明：缺电子基团3,5-二硝基苯甲酰氯与EPA、DHA形成电子转移络合物，电子转移络合物作用是一种弱的分子间作用力。
위심조종어유중분리고순도이십탄오희산（EPA）화이십이탄륙희산（DHA）적경유효방법，분별이3,5-이초기분갑선록화2-록을순수식적취갑기병희산갑지(PMDVB)수지미구，용작고효액상색보(HPLC)적고정상。균비수식전적PMDVB수지미구분리효과호，비Waters반상C18건합규효주분리인자대，주용량고。이3,5-이초기분갑선록수식적PMDVB수지효과최이상，이2-록을순수식적PMDVB수지미구，조개저，공업상구유추엄개치。작자대분리궤리적연구표명：결전자기단3,5-이초기분갑선록여EPA、DHA형성전자전이락합물，전자전이락합물작용시일충약적분자간작용력。
Porous PMDVB resin modified with electron-deficient 3, 5-dinitrobenzoylchloride and 2-chloride ethanol was prepared and applied as high-performance liquid chromatography (HPLC) stationary phases for the separation of EPA and DHA from fish oil. Satisfied separation results were obtained. Its column loading capacity, separation factor and column life were better than those of waters reverse-phase column. When porous PMDVB resin modified with 3, 5-dinitro benzoylchloride was applied as a stationary phase, the most desirable separation results were obtained. Porous PMDVB resin modified with 2-chloride ethanol is cheap. So it is very possible for large-scale application to separate EPA and DHA in industry. Discuss the separation mechanism, we found that as electron-lacked group, 3,5-dinitro benzoylchloride combinated to EPA and DHA with tran-electron complex action, which is the weak action among molecular.