CAJ | 학술논문

目的：研究空肠弯曲菌(Cj)的主要外膜蛋白(momp)基因的分子分型。方法：采用PCR方法对163株空肠弯曲菌的主要外膜蛋白基因进行扩增，用HindⅢ，HaeⅢ，MboⅡ和HhaI四种内切酶消化后进行电泳分析，并与热稳定性血清型(HS)和鞭毛蛋白A基因(flagellin A gene，fal A)分型方法相比较。结果：共观察到8种不同的主要外膜蛋白的限制性片段长度多态性的基因型，其中主要外膜蛋白基因-3(momp-3)占61％。Guillain-Barré综合征(GBS)和腹泻患者所有34株HS-19:Cj(fla)-1菌株均属同一个单一基因型，即主要外膜蛋白基因-1(momp-1)。结论：HS-19：Cj(fla)-1:momp-1菌株是空肠弯曲菌的一个特殊类型，与HS-19相关的GBS可能是阐明GBS发病机制最好的模型。
목적：연구공장만곡균(Cj)적주요외막단백(momp)기인적분자분형。방법：채용PCR방법대163주공장만곡균적주요외막단백기인진행확증，용HindⅢ，HaeⅢ，MboⅡ화HhaI사충내절매소화후진행전영분석，병여열은정성혈청형(HS)화편모단백A기인(flagellin A gene，fal A)분형방법상비교。결과：공관찰도8충불동적주요외막단백적한제성편단장도다태성적기인형，기중주요외막단백기인-3(momp-3)점61％。Guillain-Barré종합정(GBS)화복사환자소유34주HS-19:Cj(fla)-1균주균속동일개단일기인형，즉주요외막단백기인-1(momp-1)。결론：HS-19：Cj(fla)-1:momp-1균주시공장만곡균적일개특수류형，여HS-19상관적GBS가능시천명GBS발병궤제최호적모형。
Objective: To study thd momecular typing of the major outer membrane protein gene(momp) of Campylobacter jejuni(C.jejuni). Methods: The C. jejuni major outer membrane protein gene was arnplified by PCR from a total of 163 strains. AfterHind Ⅲ , HaeⅢ, Mbo Ⅱ and HhaI restriction enzyme digestions, the digest was analyzed by electrophoresis, and then comparedwith heat-stable(HS) serotype and PCR-based restriction fragnent length polymorphism(RFLP) typing of flagellin A gene (flaA). Results: Eight distinct momp RFLP genotypes were observed. Among all of them, one major type (momp-3) accounted for61% of all isolates. All 34 HS-19:Cj(fla)-1 strains,including isolates from patients with Guillain-Barré syndrome(GBS) and en-teritis,belonged to a single genotype(momp-1). Conclusion: The HS-19:Cj(fla)-1 :momp-1 strain is distinctive among C.jejunistains,and HS-19 related GBS may be an excellent model to elucidate the pathogenesis of GBS.