CAJ | 학술논문

目的研究趋化素样因子1(CKLF1)对造血功能的调节作用。方法利用MTT法，检测CKLF1真核细胞表达载体转染上清在液体培养基中，对人低密度骨髓细胞和小鼠骨髓细胞的增殖调控作用；并在半固体培养基中，观察其对人低密度骨髓细胞集落形成的刺激作用。结果 COS-7细胞表达的CKLF1能明显促进人和小鼠骨髓细胞的增殖，并能促进人骨髓细胞的集落形成，与GM-CSF有协同刺激作用。结论 CKLF1能促进人和小鼠骨髓造血干/祖细胞的增殖和分化。
목적연구추화소양인자1(CKLF1)대조혈공능적조절작용。방법이용MTT법，검측CKLF1진핵세포표체재체전염상청재액체배양기중，대인저밀도골수세포화소서골수세포적증식조공작용；병재반고체배양기중，관찰기대인저밀도골수세포집락형성적자격작용。결과 COS-7세포표체적CKLF1능명현촉진인화소서골수세포적증식，병능촉진인골수세포적집락형성，여GM-CSF유협동자격작용。결론 CKLF1능촉진인화소서골수조혈간/조세포적증식화분화。
To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. Methods Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony forma tion of human hematopoietic progenitor cells was identified in semi-solid culture. Results CKLF1 has obvi ous enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. Conclusion CKLF1 can pro mote the proliferation and differentiation of bone marrow cells.