CAJ | 학술논문

背景:肢体负压治疗周围动脉闭塞性病变,具有方便、安全、无创伤和改善疼痛症状的作用.前列腺素E1可直接松弛血管平滑肌,对神经痛有良好的止痛效果.目的:观察肢体负压对周围动脉闭塞性病变模型犬中枢神经系统感觉神经纤维中前列腺素E1免疫反应阳性神经纤维的影响.设计:随机对照的动物实验.单位:南方医科大学附属珠江医院肿瘤中心,解放军第四军医大学西京医院普外三科.材料:实验于2003-04/2004-05在解放军第四军医大学西京医院动物实验室完成.健康成年杂种犬17只,随机数字表法随机分为3组,治疗组10只、非治疗组5只和正常对照组2只.方法:治疗组和非治疗组均将动物制作左后肢缺血模型,治疗组在模型制作后14 d开始行患肢负压治疗,压力为-12 kPa,持续15 min,1次/d,连续10 d;非治疗组不做负压治疗.正常对照组不干预.主要观察指标:各组均于实验开始后24 d将动物麻醉后处死,切取L1～L5的脊髓及背根神经节,3组均行脊髓及背根神经节免疫组化染色,检测前列腺素E1免疫反应阳性纤维平均灰度值.结果:犬17只全部进入结果分析.非治疗组、治疗组和正常对照组脊髓前列腺素E1免疫反应阳性纤维平均灰度值分别为75.23±4.3,43.22±3.7,22.00±5.8;背根神经节前列腺素E1免疫反应阳性纤维平均灰度值分别为67.12±2.3,40.08±3.8,27.64±2.7,各组比较差异显著(P均＜0.01).结论:周围动脉闭塞性疾病发病后,远端肢体脊髓及背根神经节中的前列腺素E1免疫反应阳性神经纤维数量明显增多,可能是机体的一种自身保护机制.肢体负压疗法可缓解肢体疼痛,并能减少肢体动脉闭塞性病变伤害性刺激的传入.
배경:지체부압치료주위동맥폐새성병변,구유방편、안전、무창상화개선동통증상적작용.전렬선소E1가직접송이혈관평활기,대신경통유량호적지통효과.목적:관찰지체부압대주위동맥폐새성병변모형견중추신경계통감각신경섬유중전렬선소E1면역반응양성신경섬유적영향.설계:수궤대조적동물실험.단위:남방의과대학부속주강의원종류중심,해방군제사군의대학서경의원보외삼과.재료:실험우2003-04/2004-05재해방군제사군의대학서경의원동물실험실완성.건강성년잡충견17지,수궤수자표법수궤분위3조,치료조10지、비치료조5지화정상대조조2지.방법:치료조화비치료조균장동물제작좌후지결혈모형,치료조재모형제작후14 d개시행환지부압치료,압력위-12 kPa,지속15 min,1차/d,련속10 d;비치료조불주부압치료.정상대조조불간예.주요관찰지표:각조균우실험개시후24 d장동물마취후처사,절취L1～L5적척수급배근신경절,3조균행척수급배근신경절면역조화염색,검측전렬선소E1면역반응양성섬유평균회도치.결과:견17지전부진입결과분석.비치료조、치료조화정상대조조척수전렬선소E1면역반응양성섬유평균회도치분별위75.23±4.3,43.22±3.7,22.00±5.8;배근신경절전렬선소E1면역반응양성섬유평균회도치분별위67.12±2.3,40.08±3.8,27.64±2.7,각조비교차이현저(P균＜0.01).결론:주위동맥폐새성질병발병후,원단지체척수급배근신경절중적전렬선소E1면역반응양성신경섬유수량명현증다,가능시궤체적일충자신보호궤제.지체부압요법가완해지체동통,병능감소지체동맥폐새성병변상해성자격적전입.
BACKGROUND: Negative pressure of limbs is a convenient, safe and unwound way to treat peripheral arterial occlusion and to relieve pain.Prostaglandin E1 can directly rellax vascular smooth muscles and relieve pain on nerve.OBJECTIVE: To observe the effect of negative pressure of limbs immunologic reaction positive nerve fiber of prostaglandin E1 in sensory nerve fiber of central nervous system of dogs with peripheral arterial occlusion.DESIGN: Randomized controlled animalstudy.SETTING: Tumor Center of Zhujiang Hospital affiliated to Southern Medical University, the Third General Surgery of Xijing Hospital affiliated to the Fourth Military Medical University of Chinese PLA.MATERIALS: The experiment was carried out in the Animal Laboratory of Xijing Hospital affiliated to the Fourth Military Medical University of Chinese PLA from April 2003 to May 2004. A total of 17 healthy adult hybrid dogs were randomly divided into three groups: treatment group (n=10),non-treatment group (n=5) and normal control group (n=2), according to randomly digital table.METHODS: Ischemic models of left hindlimb were established in treatment group and non-treatment group. Fourteen days later, dogs in treatment group were given negative pressure (-12kPa) treatment for 15 minutes. The negative pressure was done once a day for 10 successive days.However, negative pressure was not done in non-treatment group. Animals were not interfered in normal control group.MAIN OUTCOME MEASURES: Twenty-four days later, dogs in each group were anesthetized and sacrificed. L1-L5 spinal cord and ganglia of dorsal root were selected and stained with immunohistochemical method to detect average giay value of immunologic reaction positive nerve fiber of prostaglandin E 1.RESULTS: A total of 17 dogs were involved in the final analysis. Average gray values of immunologic reaction positive nerve fiber of prostaglandin E1 in spinal cord were 75.23±4.3 in non-treatment group, 43.22±3.7 in treatment group and 22.00±5.8 in normal control group; average gray values of immunologic reaction positive nerve fiber of prostaglandin E1 in ganglia of dorsal root were 67.12±2.3, 40.08±3.8, 27.64±2.7, respectively.There was no significant difference among the three groups (P＜0.01).CONCLUSION: After onset of peripheral arterial occlusion, amount of immunologic reaction positive nerve fiber of prostaglandin E1 in spinal cord and ganglia of dorsal root of distal limbs is increased remarkably, and this may be a kink of auto-protective mechanism of organism. Negative pressure can relieve pain of limbs and decrease damaged-stimulated transmission of peripheral arterial occlusion.