现代口腔医学杂志
現代口腔醫學雜誌
현대구강의학잡지
JOURNAL OF MODERN STOMATOLOGY
2009年
6期
631-633
,共3页
王亦菁%宋九余%金岩%史俊南
王亦菁%宋九餘%金巖%史俊南
왕역정%송구여%금암%사준남
牙髓干细胞%三维培养%骨形成蛋白(BMP-2)%碱性磷酸酶ALP
牙髓榦細胞%三維培養%骨形成蛋白(BMP-2)%堿性燐痠酶ALP
아수간세포%삼유배양%골형성단백(BMP-2)%감성린산매ALP
Dental pulp stem cells%3-dimension culture%BMP-2%ALP
目的 建立大鼠牙髓干细胞(DPSC)体外培养体系及分析研究细胞因子骨形成蛋白(BMP-2)对其生物学特性的影响.方法 利用一步离心法形成细胞团块或聚集物建立DPSC三维球状培养体系,观察细胞在培养过程中形态和细胞基质合成的变化,并分析研究细胞因子BMP-2对于三维培养DPSC增殖和分化的影响.结果 三维培养的DPSC数目无明显增加,细胞基质沉积,ALP的含量增加,活性增高;在三维培养体系中,BMP能够增加ALP含量,且呈浓度依赖性.结论 三维培养体系中形成的细胞外基质作为天然的支架,由BMP-2持续释放和保留,对今后的龋齿和牙髓治疗的临床应用有一定价值.
目的 建立大鼠牙髓榦細胞(DPSC)體外培養體繫及分析研究細胞因子骨形成蛋白(BMP-2)對其生物學特性的影響.方法 利用一步離心法形成細胞糰塊或聚集物建立DPSC三維毬狀培養體繫,觀察細胞在培養過程中形態和細胞基質閤成的變化,併分析研究細胞因子BMP-2對于三維培養DPSC增殖和分化的影響.結果 三維培養的DPSC數目無明顯增加,細胞基質沉積,ALP的含量增加,活性增高;在三維培養體繫中,BMP能夠增加ALP含量,且呈濃度依賴性.結論 三維培養體繫中形成的細胞外基質作為天然的支架,由BMP-2持續釋放和保留,對今後的齲齒和牙髓治療的臨床應用有一定價值.
목적 건립대서아수간세포(DPSC)체외배양체계급분석연구세포인자골형성단백(BMP-2)대기생물학특성적영향.방법 이용일보리심법형성세포단괴혹취집물건립DPSC삼유구상배양체계,관찰세포재배양과정중형태화세포기질합성적변화,병분석연구세포인자BMP-2대우삼유배양DPSC증식화분화적영향.결과 삼유배양적DPSC수목무명현증가,세포기질침적,ALP적함량증가,활성증고;재삼유배양체계중,BMP능구증가ALP함량,차정농도의뢰성.결론 삼유배양체계중형성적세포외기질작위천연적지가,유BMP-2지속석방화보류,대금후적우치화아수치료적림상응용유일정개치.
Objective To establish the model of 3-dimension culture model of rat DPSCs and to investigate the effects of BMP-2 on the biological characteristics of the model.Methods Three-dimension globular culture model was established by cell aggregate with one step centrifugalization. Cell shape and cell matrix were observed during culture procedure. The effects of BMP-2 on the proliferation and differentiation of DPSCs in the 3-dimension culture were analyzed.Results The number of DPSCs in the 3-dimension culture did not increase. It was found that cell matrix deposited. The content of ALP and the activity of ALP were both increased. BMP increased the content of ALP in a dose-dependent manner.Conclusion The natural supporter, which is formed by the extracellular matrix in the 3-dimension culture model with facilitation of BMP, will be valuable for the clinical application to pulp disease.
