CAJ | 학술논문

利用含1%TritonX-100和0.1%SDS的提取缓冲液提取河蟹(Eriocheir sinensis)精子膜蛋白.对所得的精子膜蛋白溶液进行SDS-PAGE电泳分析.结果显示,河蟹的精子含有12种不同的膜蛋白组分.对这些精子膜蛋白组分的生化特性进行分析,结果表明,河蟹精子膜蛋白是1组呈酸性的低分子量糖蛋白,分子量为21.6～75.5 kD,等电点在4.8左右.
이용함1%TritonX-100화0.1%SDS적제취완충액제취하해(Eriocheir sinensis)정자막단백.대소득적정자막단백용액진행SDS-PAGE전영분석.결과현시,하해적정자함유12충불동적막단백조분.대저사정자막단백조분적생화특성진행분석,결과표명,하해정자막단백시1조정산성적저분자량당단백,분자량위21.6～75.5 kD,등전점재4.8좌우.
Sperm membrane proteins of Eriocheir sinensis are extracted with extraction buffer containing 1%TritonX-100 and 0.1%SDS.Then the proteins were analyzed by SDS-PAGE.Results show the sperm membrane proteins of E sinensis include 12 different kinds of proteins.Biochemical characteristics of these sperm membrane proteins are analyzed,and the results show that their molecular weights are between 21.6 kD and 75.5 kD,and the isoelectric points(PI)are about 4.8.which indicates the sperm membrane proteins of E sinensis are a set of acid glycoproteins with low molecularweights.