CAJ | 학술논문

目的:探讨一种有效的记录人心房肌细胞小电导钙激活钾通道(small conductance Ca2+-activated K+ channels,SK)电流的全细胞膜片钳方法.方法:以人心房肌细胞为研究对象,采用传统全细胞膜片钳技术,记录人心房肌细胞的小电导钙激活钾通道的宏观电流.传统全细胞模式形成后,分别记录在浴液中加入apamin(100nM)前后的电流,两者相减就得到了apamin敏感的SK2的电流.结果:采用传统全细胞膜片钳技术,能有效记录人心房肌细胞上apamin敏感的宏观电流.该电流是内向整流,去极化激活时,无明显失活现象,无尾电流出现,这些都符合SK2电流特性.结论:本实验提供了一种简单、有效的记录入心房肌细胞SK2宏观电流的方法,为今后深入的研究提供了实验基础.
목적:탐토일충유효적기록인심방기세포소전도개격활갑통도(small conductance Ca2+-activated K+ channels,SK)전류적전세포막편겸방법.방법:이인심방기세포위연구대상,채용전통전세포막편겸기술,기록인심방기세포적소전도개격활갑통도적굉관전류.전통전세포모식형성후,분별기록재욕액중가입apamin(100nM)전후적전류,량자상감취득도료apamin민감적SK2적전류.결과:채용전통전세포막편겸기술,능유효기록인심방기세포상apamin민감적굉관전류.해전류시내향정류,거겁화격활시,무명현실활현상,무미전류출현,저사도부합SK2전류특성.결론:본실험제공료일충간단、유효적기록입심방기세포SK2굉관전류적방법,위금후심입적연구제공료실험기출.
Objective: To establish an effective and stable method of recording small conductance Ca2+-activated K+ Channel (SK) currents in human atrial myocytes by conventional whole-cell patch clamp technique.Methods: Conventional whole-cell patch clamp technique was used to record apamin-sensitive currents in isolated right atrial myocytes from human right appendage. After conventional whole-cell configuration was made, apamin (100 nM)-a special blocker of SK2 current was added into extracellular solution. The apamin-sensitive current was obtained by using digital subtraction after apamin from control. Results: SK2 currents in human atrial myocytes were effectively recorded. SK2 current was inward rectification and not obvious effect of inactivation.Conclusion: Conventional whole-cell patch clamp technique can be used to record the SK2 currents. This method provides a basis for future research.