山东化工
山東化工
산동화공
SHANDONG CHEMICAL INDUSTRY
2011年
9期
8-12,19
,共6页
吕世锋%任建伟%张满%杨付伟%阎振丽
呂世鋒%任建偉%張滿%楊付偉%閻振麗
려세봉%임건위%장만%양부위%염진려
黑曲霉%木聚糖酶%酶活力%培养基%发酵条件%响应面
黑麯黴%木聚糖酶%酶活力%培養基%髮酵條件%響應麵
흑곡매%목취당매%매활력%배양기%발효조건%향응면
aspergillus niger%xylanase%enzyme activity%culture medium%fermentation condition%response surface
通过单因素实验及响应面方法对黑曲霉菌株产木聚糖酶发酵条件进行了研究，结果表明：当培养基为麸皮40g／L，（NH4）2SO4 4g／L，KH2PO4 3g／L，MgSO4 0．8g／L，吐温801g／L；初始pH值5．0，接种量8．0％，培养温度30℃；此条件下发酵木聚糖酶酶活达1456．271U／mL。
通過單因素實驗及響應麵方法對黑麯黴菌株產木聚糖酶髮酵條件進行瞭研究，結果錶明：噹培養基為麩皮40g／L，（NH4）2SO4 4g／L，KH2PO4 3g／L，MgSO4 0．8g／L，吐溫801g／L；初始pH值5．0，接種量8．0％，培養溫度30℃；此條件下髮酵木聚糖酶酶活達1456．271U／mL。
통과단인소실험급향응면방법대흑곡매균주산목취당매발효조건진행료연구，결과표명：당배양기위부피40g／L，（NH4）2SO4 4g／L，KH2PO4 3g／L，MgSO4 0．8g／L，토온801g／L；초시pH치5．0，접충량8．0％，배양온도30℃；차조건하발효목취당매매활체1456．271U／mL。
The fermentation conditions of aspergillus niger producing xylanase were studied by single facter experiment and response surface method. The results were as follows ： bran 40 g / L , （ NH4 ） 2 SO4 4g/L, KH2PO4 4g/L, MgSO4 0.8g/L, Tween 80 1 g/L, initial pH value 5.0, inoculation volume 8.0%, temperature 30℃, under these conditions, the activity of xylanase was reached 1456.27IU/mL.
